JAK2V617F mutation and spontaneous megakaryocytic or erythroid colony formation in patients with essential thrombocythaemia (ET) or polycythaemia vera (PV)

Satu Mustjoki, Ioana Borze, Terra L. Lasho, Riitta Alitalo, Animesh D Pardanani, Sakari Knuutila, Eeva Juvonen

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The in vitro cultures of erythroid (BFU-E) and megakaryocytic (CFU-Meg) progenitors have been useful diagnostic tools in myeloproliferative disorders (MPD). However, after the discovery of the JAK2V617F mutation, their diagnostic role has been uncertain. In this single-centre retrospective study we analyzed JAK2V617F mutation in 58 ET and 42 PV patients diagnosed according to WHO criteria and compared the results with those of colony forming assays with special emphasis on CFU-Meg growth. 91% of PV and 57% of ET patients had JAK2V617F mutation and they all showed spontaneous BFU-E growth. However, endogenous BFU-E formation was also seen in nine JAK2V617F mutation negative patients displaying also a normal JAK2 exon 12 allele. Endogeneous CFU-Meg colony formation was found in 59% of PV and 53% of the ET patients. A subgroup of ET patients (n = 7) displayed sole spontaneous CFU-Meg growth without spontaneous BFU-E growth. They all were JAK2 mutation negative, but one of them had MPL mutation. In conclusion, in vitro cultures of haematopoietic progenitors are sensitive diagnostic tools in the present group of 100 MPD patients revealing also JAK2 mutation negative ET and PV patients displaying sole spontaneous CFU-Meg or BFU-E growth.

Original languageEnglish (US)
Pages (from-to)54-59
Number of pages6
JournalLeukemia Research
Volume33
Issue number1
DOIs
StatePublished - Jan 2009

Fingerprint

Essential Thrombocythemia
Polycythemia Vera
Erythroid Precursor Cells
Mutation
Growth
Myeloproliferative Disorders
Exons
Retrospective Studies
Alleles

Keywords

  • Colony formation
  • Diagnostic tool
  • ET
  • JAK2
  • PV

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

Cite this

JAK2V617F mutation and spontaneous megakaryocytic or erythroid colony formation in patients with essential thrombocythaemia (ET) or polycythaemia vera (PV). / Mustjoki, Satu; Borze, Ioana; Lasho, Terra L.; Alitalo, Riitta; Pardanani, Animesh D; Knuutila, Sakari; Juvonen, Eeva.

In: Leukemia Research, Vol. 33, No. 1, 01.2009, p. 54-59.

Research output: Contribution to journalArticle

Mustjoki, Satu ; Borze, Ioana ; Lasho, Terra L. ; Alitalo, Riitta ; Pardanani, Animesh D ; Knuutila, Sakari ; Juvonen, Eeva. / JAK2V617F mutation and spontaneous megakaryocytic or erythroid colony formation in patients with essential thrombocythaemia (ET) or polycythaemia vera (PV). In: Leukemia Research. 2009 ; Vol. 33, No. 1. pp. 54-59.
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