TY - JOUR
T1 - Isolation of myosin heavy chain from small skeletal muscle samples by preparative continuous elution gel electrophoresis
T2 - Application to measurement of synthesis rate in human and animal tissue
AU - Balagopal, P.
AU - Nair, K. Sreekumaran
AU - Stirewalt, William S.
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1994/8/15
Y1 - 1994/8/15
N2 - A convenient procedure for the isolation of milligram quantities of myosin heavy chain (MHC) from small samples of muscle tissue using preparative gel electrophoresis is described. Application of the methodology to measurements of the fractional synthesis rates (ks) of MHC using skeletal muscle biopsy samples from humans and cardiac tissue from pigs following continuous intravenous infusions of stable isotope (L-[1-13C]-leucine) is demonstrated. The sensitivity and reproducibility of the determination of stable isotope enrichment are also defined. In addition, measurements of the ks of MHC in skeletal muscle of rats in vivo using radioisotope-tracer methodologies are described.
AB - A convenient procedure for the isolation of milligram quantities of myosin heavy chain (MHC) from small samples of muscle tissue using preparative gel electrophoresis is described. Application of the methodology to measurements of the fractional synthesis rates (ks) of MHC using skeletal muscle biopsy samples from humans and cardiac tissue from pigs following continuous intravenous infusions of stable isotope (L-[1-13C]-leucine) is demonstrated. The sensitivity and reproducibility of the determination of stable isotope enrichment are also defined. In addition, measurements of the ks of MHC in skeletal muscle of rats in vivo using radioisotope-tracer methodologies are described.
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U2 - 10.1006/abio.1994.1381
DO - 10.1006/abio.1994.1381
M3 - Article
C2 - 7985807
AN - SCOPUS:0028021597
SN - 0003-2697
VL - 221
SP - 72
EP - 77
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 1
ER -