Isolation of myosin heavy chain from small skeletal muscle samples by preparative continuous elution gel electrophoresis: Application to measurement of synthesis rate in human and animal tissue

P. Balagopal, K. Sreekumaran Nair, William S. Stirewalt

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

A convenient procedure for the isolation of milligram quantities of myosin heavy chain (MHC) from small samples of muscle tissue using preparative gel electrophoresis is described. Application of the methodology to measurements of the fractional synthesis rates (ks) of MHC using skeletal muscle biopsy samples from humans and cardiac tissue from pigs following continuous intravenous infusions of stable isotope (L-[1-13C]-leucine) is demonstrated. The sensitivity and reproducibility of the determination of stable isotope enrichment are also defined. In addition, measurements of the ks of MHC in skeletal muscle of rats in vivo using radioisotope-tracer methodologies are described.

Original languageEnglish (US)
Pages (from-to)72-77
Number of pages6
JournalAnalytical Biochemistry
Volume221
Issue number1
DOIs
StatePublished - Aug 15 1994

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Isolation of myosin heavy chain from small skeletal muscle samples by preparative continuous elution gel electrophoresis: Application to measurement of synthesis rate in human and animal tissue'. Together they form a unique fingerprint.

Cite this