Isolation of mutant T lymphocytes with defects in capacitative calcium entry

Andrew T. Serafini, Richard S. Lewis, Neil A. Clipstone, Richard J Bram, Christopher Fanger, Steve Flering, Leonard A. Herzenberg, Gerald R. Crabtree

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Calcium and calcium-binding proteins play important roles in the signaling cascade leading from the initial engagement of TCRs on T cells to the fully activated state. To undertake a molecular dissection of this cascade, we first isolated a Jurkat T cell line derivative containing the NF-AT promoter element driving transcription of the diphtheria toxin A chain gene (dipA), resulting in rapid cell death. Selecting viable cells that fail to activate NF-AT-dependent transcription, we isolated two independent cell lines possessing defects in capacitative Ca2+ entry. NF-AT-dependent transcription can be restored in these cells by expression of a constitutively active calcineurin, but not by overexpression of the Ca2+ regulatory protein CAML, which can normally replace the Ca2+ signal. The defect in these cell lines probably lies between CAML and calcineurin in the T cell activation cascade.

Original languageEnglish (US)
Pages (from-to)239-250
Number of pages12
JournalImmunity
Volume3
Issue number2
DOIs
StatePublished - 1995
Externally publishedYes

Fingerprint

Calcineurin
Calcium
T-Lymphocytes
Cell Line
Calcium-Binding Proteins
Jurkat Cells
Dissection
Cell Death
Genes
Proteins
diphtheria toxin fragment A

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Infectious Diseases

Cite this

Serafini, A. T., Lewis, R. S., Clipstone, N. A., Bram, R. J., Fanger, C., Flering, S., ... Crabtree, G. R. (1995). Isolation of mutant T lymphocytes with defects in capacitative calcium entry. Immunity, 3(2), 239-250. https://doi.org/10.1016/1074-7613(95)90093-4

Isolation of mutant T lymphocytes with defects in capacitative calcium entry. / Serafini, Andrew T.; Lewis, Richard S.; Clipstone, Neil A.; Bram, Richard J; Fanger, Christopher; Flering, Steve; Herzenberg, Leonard A.; Crabtree, Gerald R.

In: Immunity, Vol. 3, No. 2, 1995, p. 239-250.

Research output: Contribution to journalArticle

Serafini, AT, Lewis, RS, Clipstone, NA, Bram, RJ, Fanger, C, Flering, S, Herzenberg, LA & Crabtree, GR 1995, 'Isolation of mutant T lymphocytes with defects in capacitative calcium entry', Immunity, vol. 3, no. 2, pp. 239-250. https://doi.org/10.1016/1074-7613(95)90093-4
Serafini, Andrew T. ; Lewis, Richard S. ; Clipstone, Neil A. ; Bram, Richard J ; Fanger, Christopher ; Flering, Steve ; Herzenberg, Leonard A. ; Crabtree, Gerald R. / Isolation of mutant T lymphocytes with defects in capacitative calcium entry. In: Immunity. 1995 ; Vol. 3, No. 2. pp. 239-250.
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