Isolation of extracellular RNA from bile

Irene K. Yan, Valentin X. Berdah, Tushar C Patel

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The study of extracellular RNA has been recently reported as a tool for biomarker discovery. Extracellular vesicles can be isolated from different types of body fluids which contain protein, mRNA, and noncoding RNA. Extracellular RNA isolated from bile could be a useful tool for analyzing biliary tract diseases or cancer. Herein, we describe protocols based on modifications of commercially available kits for the collection, processing, and isolation of extracellular RNA from bile.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages59-67
Number of pages9
Volume1740
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1740
ISSN (Print)1064-3745

Fingerprint

Bile
RNA
Biliary Tract Neoplasms
Biliary Tract Diseases
Untranslated RNA
Body Fluids
Biomarkers
Messenger RNA
Proteins
Extracellular Vesicles

Keywords

  • Bile
  • Exosomes
  • Extracellular RNA
  • Extracellular Vesicles
  • Isolation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Yan, I. K., Berdah, V. X., & Patel, T. C. (2018). Isolation of extracellular RNA from bile. In Methods in Molecular Biology (Vol. 1740, pp. 59-67). (Methods in Molecular Biology; Vol. 1740). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7652-2_6

Isolation of extracellular RNA from bile. / Yan, Irene K.; Berdah, Valentin X.; Patel, Tushar C.

Methods in Molecular Biology. Vol. 1740 Humana Press Inc., 2018. p. 59-67 (Methods in Molecular Biology; Vol. 1740).

Research output: Chapter in Book/Report/Conference proceedingChapter

Yan, IK, Berdah, VX & Patel, TC 2018, Isolation of extracellular RNA from bile. in Methods in Molecular Biology. vol. 1740, Methods in Molecular Biology, vol. 1740, Humana Press Inc., pp. 59-67. https://doi.org/10.1007/978-1-4939-7652-2_6
Yan IK, Berdah VX, Patel TC. Isolation of extracellular RNA from bile. In Methods in Molecular Biology. Vol. 1740. Humana Press Inc. 2018. p. 59-67. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7652-2_6
Yan, Irene K. ; Berdah, Valentin X. ; Patel, Tushar C. / Isolation of extracellular RNA from bile. Methods in Molecular Biology. Vol. 1740 Humana Press Inc., 2018. pp. 59-67 (Methods in Molecular Biology).
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