Isolation of extracellular RNA from bile

Irene K. Yan, Valentin X. Berdah, Tushar C Patel

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The study of extracellular RNA has been recently reported as a tool for biomarker discovery. Extracellular vesicles can be isolated from different types of body fluids which contain protein, mRNA, and noncoding RNA. Extracellular RNA isolated from bile could be a useful tool for analyzing biliary tract diseases or cancer. Herein, we describe protocols based on modifications of commercially available kits for the collection, processing, and isolation of extracellular RNA from bile.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages59-67
Number of pages9
Volume1740
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1740
ISSN (Print)1064-3745

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Keywords

  • Bile
  • Exosomes
  • Extracellular RNA
  • Extracellular Vesicles
  • Isolation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Yan, I. K., Berdah, V. X., & Patel, T. C. (2018). Isolation of extracellular RNA from bile. In Methods in Molecular Biology (Vol. 1740, pp. 59-67). (Methods in Molecular Biology; Vol. 1740). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7652-2_6