Isolation of extracellular nanovesicle microRNA from liver cancer cells in culture

Takayuki Kogure, Tushar C Patel

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

MicroRNA can be transferred across cells within extracellular vesicles such as exosomes. In order to analyze the biological effects of extracellular vesicle microRNA, it is necessary to isolate these vesicles and to extract their miRNA content.Here, we describe an approach to the isolation of cellular nanovesicles from liver cancer cell lines that can be used for the isolation of RNA and microRNA.

Original languageEnglish (US)
Pages (from-to)11-18
Number of pages8
JournalMethods in molecular biology (Clifton, N.J.)
Volume1024
DOIs
StatePublished - 2013

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Liver Neoplasms
MicroRNAs
Cell Culture Techniques
Exosomes
RNA
Cell Line
Extracellular Vesicles

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Isolation of extracellular nanovesicle microRNA from liver cancer cells in culture. / Kogure, Takayuki; Patel, Tushar C.

In: Methods in molecular biology (Clifton, N.J.), Vol. 1024, 2013, p. 11-18.

Research output: Contribution to journalArticle

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