Isolation of extracellular nanovesicle microRNA from liver cancer cells in culture

Takayuki Kogure, Tushar Patel

Research output: Chapter in Book/Report/Conference proceedingChapter

18 Scopus citations

Abstract

MicroRNA can be transferred across cells within extracellular vesicles such as exosomes. In order to analyze the biological effects of extracellular vesicle microRNA, it is necessary to isolate these vesicles and to extract their miRNA content. Here, we describe an approach to the isolation of cellular nanovesicles from liver cancer cell lines that can be used for the isolation of RNA and microRNA.

Original languageEnglish (US)
Title of host publicationCirculating MicroRNAs
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages11-18
Number of pages8
ISBN (Print)9781627034524
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume1024
ISSN (Print)1064-3745

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Keywords

  • Centrifugation
  • Exosomes
  • Hepatocellular cancer
  • MicroRNA
  • Nanovesicles
  • RNA isolation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Kogure, T., & Patel, T. (2013). Isolation of extracellular nanovesicle microRNA from liver cancer cells in culture. In Circulating MicroRNAs: Methods and Protocols (pp. 11-18). (Methods in Molecular Biology; Vol. 1024). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-453-1_2