Isolation and rapid identification of an abundant self-peptide from class II HLA-DRB1*0401 alleles induced by measles vaccine virus infection

Inna G. Ovsyannikova, Kenneth L. Johnson, Stephen Naylor, Gregory A. Poland

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Class II HLA-DR genes play an important role in the immune response to viral antigens. The effect of measles vaccine virus (MVV) infection on the induction of self-peptides presented by HLA-DR molecules during the immune response to viral infection is poorly known. Here, we describe a strategy for isolation and rapid sequence determination of an MVV-inducible class II bound peptide from a membrane protein (Leu-13). Peptides bound to HLA-DR4 (DRB1*0401 peptide complex) were eluted from immunoaffinity-purified HLA-DR4, peptides were differentially screened by MALDI-TOF-MS and subsequently sequenced by post source decay (PSD)-MALDI-TOF-MS. Human B-cells infected with MVV demonstrated an enhanced pattern of self-peptide production after MVV infection. This relatively simple analytical protocol provides a sensitive method for the direct identification of peptides associated with MHC class II DR molecules. More broadly, this same approach can be used to identify sequences of specific MVV processed peptides presented by any class II MHC DR molecule. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)1-12
Number of pages12
JournalJournal of Immunological Methods
Volume246
Issue number1-2
DOIs
StatePublished - Dec 1 2000

Keywords

  • HLA-DR
  • Mass spectrometry
  • Measles vaccine virus
  • Peptides

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'Isolation and rapid identification of an abundant self-peptide from class II HLA-DRB1*0401 alleles induced by measles vaccine virus infection'. Together they form a unique fingerprint.

Cite this