Isolation and electrophoresis of nuclear proteins of bean

Thomas C. Spelsberg, Igor V. Sarkissian

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Methods are described for the fractionation of the acidic and basic proteins associated with the chromatin of plant tissue. Polyacrylamide gel systems are also described which give optimal electrophoretic resolution of these protein fractions. Utilizing these techniques, comparative studies revealed a differential specificity of these proteins for the undifferentiated hook and the more differentiated lower hypocotyl tissue of bean hypocotyls. The acidic nuclear protein fractions exhibited a difference in gel patterns of the two stages of development while the histones showed little difference. The ratio of the ethanol-HCl extracted histones to the HCl extracted histones appeared to increase with development.

Original languageEnglish (US)
Pages (from-to)2083-2088
Number of pages6
JournalPhytochemistry
Volume7
Issue number12
StatePublished - Dec 1968
Externally publishedYes

Fingerprint

nuclear proteins
Nuclear Proteins
Electrophoresis
histones
Histones
electrophoresis
Hypocotyl
beans
hypocotyls
gels
Tissue
Proteins
proteins
Hooks
polyacrylamide
Fractionation
Chromatin
chromatin
plant tissues
fractionation

ASJC Scopus subject areas

  • Drug Discovery
  • Organic Chemistry
  • Molecular Biology
  • Biochemistry
  • Plant Science

Cite this

Spelsberg, T. C., & Sarkissian, I. V. (1968). Isolation and electrophoresis of nuclear proteins of bean. Phytochemistry, 7(12), 2083-2088.

Isolation and electrophoresis of nuclear proteins of bean. / Spelsberg, Thomas C.; Sarkissian, Igor V.

In: Phytochemistry, Vol. 7, No. 12, 12.1968, p. 2083-2088.

Research output: Contribution to journalArticle

Spelsberg, TC & Sarkissian, IV 1968, 'Isolation and electrophoresis of nuclear proteins of bean', Phytochemistry, vol. 7, no. 12, pp. 2083-2088.
Spelsberg TC, Sarkissian IV. Isolation and electrophoresis of nuclear proteins of bean. Phytochemistry. 1968 Dec;7(12):2083-2088.
Spelsberg, Thomas C. ; Sarkissian, Igor V. / Isolation and electrophoresis of nuclear proteins of bean. In: Phytochemistry. 1968 ; Vol. 7, No. 12. pp. 2083-2088.
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