Isolation and comparative mapping of a human chromosome 20-specific α-satellite DNA clone

A. Baldini, N. Archidiacono, R. Carbone, A. Bolino, Vijayalakshmi Shridhar, O. J. Miller, D. A. Miller, D. C. Ward, M. Rocchi

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

We have isolated and characterized a human genomic DNA clone (PZ20, locus D20Z2) that identifies, under high-stringency hybridization conditions, an alphoid DNA subset specific for chromosome 20. The specificity was determined using fluorescence in situ hybridization. Sequence analysis confirmed our previously reported data on the great similarity between the chromosome 20 and chromosome 2 alphoid subsets. Comparative mapping of pZ20 on chimpanzee and gorilla chromosomes, also performed under high-stringency conditions, indicates that the alphoid subset has ancestral sequences on chimpanzee chromosome 11 and gorilla chromosome 19. However, no hybridization was observed to chromosomes 21 in the great apes, the homolog of human chromosome 20.

Original languageEnglish (US)
Pages (from-to)12-16
Number of pages5
JournalCytogenetics and Cell Genetics
Volume59
Issue number1
StatePublished - 1992
Externally publishedYes

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Chromosomes, Human, Pair 20
Satellite DNA
Human Chromosomes
Gorilla gorilla
Pan troglodytes
Clone Cells
Chromosomes, Human, Pair 19
Chromosomes, Human, Pair 21
Chromosomes, Human, Pair 11
Chromosomes, Human, Pair 2
DNA
Hominidae
Fluorescence In Situ Hybridization
Sequence Analysis
Chromosomes

ASJC Scopus subject areas

  • Cell Biology
  • Genetics

Cite this

Baldini, A., Archidiacono, N., Carbone, R., Bolino, A., Shridhar, V., Miller, O. J., ... Rocchi, M. (1992). Isolation and comparative mapping of a human chromosome 20-specific α-satellite DNA clone. Cytogenetics and Cell Genetics, 59(1), 12-16.

Isolation and comparative mapping of a human chromosome 20-specific α-satellite DNA clone. / Baldini, A.; Archidiacono, N.; Carbone, R.; Bolino, A.; Shridhar, Vijayalakshmi; Miller, O. J.; Miller, D. A.; Ward, D. C.; Rocchi, M.

In: Cytogenetics and Cell Genetics, Vol. 59, No. 1, 1992, p. 12-16.

Research output: Contribution to journalArticle

Baldini, A, Archidiacono, N, Carbone, R, Bolino, A, Shridhar, V, Miller, OJ, Miller, DA, Ward, DC & Rocchi, M 1992, 'Isolation and comparative mapping of a human chromosome 20-specific α-satellite DNA clone', Cytogenetics and Cell Genetics, vol. 59, no. 1, pp. 12-16.
Baldini, A. ; Archidiacono, N. ; Carbone, R. ; Bolino, A. ; Shridhar, Vijayalakshmi ; Miller, O. J. ; Miller, D. A. ; Ward, D. C. ; Rocchi, M. / Isolation and comparative mapping of a human chromosome 20-specific α-satellite DNA clone. In: Cytogenetics and Cell Genetics. 1992 ; Vol. 59, No. 1. pp. 12-16.
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AU - Shridhar, Vijayalakshmi

AU - Miller, O. J.

AU - Miller, D. A.

AU - Ward, D. C.

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