TY - JOUR
T1 - Isolation and characterization of two plaque size variants of Theiler's murine encephalomyelitis virus (DA strain)
AU - Oleszak, E. L.
AU - Leibowitz, J. L.
AU - Rodriguez, M.
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1988
Y1 - 1988
N2 - We have isolated two plaques size variants of Theiler's murine encephalomyelitis virus (TMEV) strain DA. One variant, TMEV-C(L) (C(L)), produced large plaques, while the other, TMEV-D(S) (D(S)) produced small plaques in L-2 cells. The D(S) variant yielded a lower titre in BHK cells and had a significantly slower growth rate when compared to C(L) and DA. In contrast, D(S) replicated to a higher titre in the central nervous system (CNS) of infected mice than the large plaque counterpart and DA. Furthermore, the D(S) (but not C(L)) variant was temperature-sensitive, replicating 130- to 500-fold more at 37°C than at 39°C. Although D(S), C(L) and DA were able to establish persistent CNS infections in mice, only the D(S) variant and DA induced demyelinating disease in SJL/J mice. Therefore persistence of TMEV in the CNS is not sufficient to produce demyelinating disease. These two variants of the DA strain of TMEV will be useful for study of the viral genetic elements important in the mechanism of virus-induced demyelination.
AB - We have isolated two plaques size variants of Theiler's murine encephalomyelitis virus (TMEV) strain DA. One variant, TMEV-C(L) (C(L)), produced large plaques, while the other, TMEV-D(S) (D(S)) produced small plaques in L-2 cells. The D(S) variant yielded a lower titre in BHK cells and had a significantly slower growth rate when compared to C(L) and DA. In contrast, D(S) replicated to a higher titre in the central nervous system (CNS) of infected mice than the large plaque counterpart and DA. Furthermore, the D(S) (but not C(L)) variant was temperature-sensitive, replicating 130- to 500-fold more at 37°C than at 39°C. Although D(S), C(L) and DA were able to establish persistent CNS infections in mice, only the D(S) variant and DA induced demyelinating disease in SJL/J mice. Therefore persistence of TMEV in the CNS is not sufficient to produce demyelinating disease. These two variants of the DA strain of TMEV will be useful for study of the viral genetic elements important in the mechanism of virus-induced demyelination.
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U2 - 10.1099/0022-1317-69-9-2413
DO - 10.1099/0022-1317-69-9-2413
M3 - Article
C2 - 2842440
AN - SCOPUS:0023755613
SN - 0022-1317
VL - 69
SP - 2413
EP - 2418
JO - Journal of General Virology
JF - Journal of General Virology
IS - 9
ER -