Isolation and characterization of two plaque size variants of Theiler's murine encephalomyelitis virus (DA strain)

E. L. Oleszak, J. L. Leibowitz, M. Rodriguez

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

We have isolated two plaques size variants of Theiler's murine encephalomyelitis virus (TMEV) strain DA. One variant, TMEV-C(L) (C(L)), produced large plaques, while the other, TMEV-D(S) (D(S)) produced small plaques in L-2 cells. The D(S) variant yielded a lower titre in BHK cells and had a significantly slower growth rate when compared to C(L) and DA. In contrast, D(S) replicated to a higher titre in the central nervous system (CNS) of infected mice than the large plaque counterpart and DA. Furthermore, the D(S) (but not C(L)) variant was temperature-sensitive, replicating 130- to 500-fold more at 37°C than at 39°C. Although D(S), C(L) and DA were able to establish persistent CNS infections in mice, only the D(S) variant and DA induced demyelinating disease in SJL/J mice. Therefore persistence of TMEV in the CNS is not sufficient to produce demyelinating disease. These two variants of the DA strain of TMEV will be useful for study of the viral genetic elements important in the mechanism of virus-induced demyelination.

Original languageEnglish (US)
Pages (from-to)2413-2418
Number of pages6
JournalJournal of General Virology
Volume69
Issue number9
DOIs
StatePublished - Jan 1 1988

ASJC Scopus subject areas

  • Virology

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