Isolation and characterization of two new functional subtypes of HLA‐B35

Graciela Theiler; Marcelo Pando; José M. Delfino; Masafumi Takiguchi; M. Leonardo Satz

doi:10.1111/j.1399-0039.1993.tb01993.x

Isolation and characterization of two new functional subtypes of HLA‐B35

Graciela Theiler, Marcelo Pando, José M. Delfino, Masafumi Takiguchi, M. Leonardo Satz

Laboratory Medicine and Pathology

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Human minor histocompatibility antigen‐specific, HLA‐B*3501‐restricted cytotoxic T‐cell clones were assayed on a panel of 25 different target cells previously typed by serology as HLA‐B35. Cells from 6 donors were not killed and cells from 2 of these were further studied by molecular cloning to characterize their HLA class I alleles. Two new HLA‐B35 subtypes were identified. The sequence of one of them differs from B*3501 by one nucleotide change at codon 156, replacing a Leu for an Arg. The sequence of the other new subtype also shows a single nucleotide change compared to B*3501, with Gly Val substitution at residue 16. With these new variants, the allelic complexity of HLA‐B35 extends now to eight subtypes. 1993 Blackwell Munksgaard

Original language	English (US)
Pages (from-to)	143-147
Number of pages	5
Journal	Tissue Antigens
Volume	41
Issue number	3
DOIs	https://doi.org/10.1111/j.1399-0039.1993.tb01993.x
State	Published - Mar 1993

Keywords

HLA‐B35 subtypes

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Biochemistry
Genetics

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10.1111/j.1399-0039.1993.tb01993.x

Cite this

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abstract = "Human minor histocompatibility antigen‐specific, HLA‐B*3501‐restricted cytotoxic T‐cell clones were assayed on a panel of 25 different target cells previously typed by serology as HLA‐B35. Cells from 6 donors were not killed and cells from 2 of these were further studied by molecular cloning to characterize their HLA class I alleles. Two new HLA‐B35 subtypes were identified. The sequence of one of them differs from B*3501 by one nucleotide change at codon 156, replacing a Leu for an Arg. The sequence of the other new subtype also shows a single nucleotide change compared to B*3501, with Gly Val substitution at residue 16. With these new variants, the allelic complexity of HLA‐B35 extends now to eight subtypes. 1993 Blackwell Munksgaard",

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AU - Theiler, Graciela

AU - Pando, Marcelo

AU - Delfino, José M.

AU - Takiguchi, Masafumi

AU - Satz, M. Leonardo

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AB - Human minor histocompatibility antigen‐specific, HLA‐B*3501‐restricted cytotoxic T‐cell clones were assayed on a panel of 25 different target cells previously typed by serology as HLA‐B35. Cells from 6 donors were not killed and cells from 2 of these were further studied by molecular cloning to characterize their HLA class I alleles. Two new HLA‐B35 subtypes were identified. The sequence of one of them differs from B*3501 by one nucleotide change at codon 156, replacing a Leu for an Arg. The sequence of the other new subtype also shows a single nucleotide change compared to B*3501, with Gly Val substitution at residue 16. With these new variants, the allelic complexity of HLA‐B35 extends now to eight subtypes. 1993 Blackwell Munksgaard

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Isolation and characterization of two new functional subtypes of HLA‐B35

Abstract

Keywords

ASJC Scopus subject areas

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