Isolation and characterization of the estrogen receptor in hen oviduct

Evidence for two molecular species

O. L. Kon, R. A. Webster, T. C. Spelsberg

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Isolation of the estrogen receptor was undertaken for comparison with the progesterone receptor with respect to seasonal variations in receptor level and function and the specificity of nuclear binding sites in the hen oviduct. The estrogen receptor in the chick and hen oviduct has been only partially characterized, with conflicting or incomplete information on its properties. We have isolated a binding protein and have partially purified it by ammonium sulfate precipitation. The binding protein displays a single, high affinity class of sites (Kd ~ 10-10 M) with a low capacity (35 fmol/mg protein) for 17β-estradiol, shows a marked specificity for estrogenic compounds, and is tissue specific. The protein sediments at approximately 8S in low salt and at approximately 4S in high salt conditions. The protein elutes from molecular sieve chromatography (agarose-0.5 m) in high salt conditions (0.3 M KCl) in a molecular weight range of approximately 60,000. DEAE chromatography and isoelectric focusing suggest that there are two molecular species, one focusing at pH 6.8 and the other at pH 7.3. Estrogen complexed to this protein will bind to nuclear acceptor sites in a cell-free assay in which free estrogen, estrogen bound to serum proteins, and [3H]estradiol receptor exchanged with a 100-fold excess of cold estradiol do not bind. Binding to nuclei is saturable and displaceable by an excess of nonradioactive estrogen receptor complexes under conditions of constant protein concentration. The above results represent the most detailed characterization to date of the cytosol estrogen receptor of hen oviduct. This is the first demonstration of saturable, receptor-dependent, cell-free binding to target organ nuclei.

Original languageEnglish (US)
Pages (from-to)1182-1191
Number of pages10
JournalEndocrinology
Volume107
Issue number4
StatePublished - 1980

Fingerprint

Oviducts
Estrogen Receptors
Estrogens
Salts
Proteins
Estradiol
Carrier Proteins
Estradiol Receptors
Ammonium Sulfate
Isoelectric Focusing
Progesterone Receptors
Cytosol
Sepharose
Gel Chromatography
Chromatography
Blood Proteins
Molecular Weight
Binding Sites

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Isolation and characterization of the estrogen receptor in hen oviduct : Evidence for two molecular species. / Kon, O. L.; Webster, R. A.; Spelsberg, T. C.

In: Endocrinology, Vol. 107, No. 4, 1980, p. 1182-1191.

Research output: Contribution to journalArticle

Kon, OL, Webster, RA & Spelsberg, TC 1980, 'Isolation and characterization of the estrogen receptor in hen oviduct: Evidence for two molecular species', Endocrinology, vol. 107, no. 4, pp. 1182-1191.
Kon, O. L. ; Webster, R. A. ; Spelsberg, T. C. / Isolation and characterization of the estrogen receptor in hen oviduct : Evidence for two molecular species. In: Endocrinology. 1980 ; Vol. 107, No. 4. pp. 1182-1191.
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