Isolation and Characterization of a x Amyloid Fibril Protein

Morie Gertz, M. SKINNER, A. S. COHEN, L. H. CONNORS, R. A. KYLE

Research output: Contribution to journalArticle

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Abstract

The fibril in primary amyloidosis (AL) is composed of a monoclonal light chain or portions thereof. No unique primary structure has been identified that predisposes certain light chains to form amyloid fibrils. Currently, classification of amyloidosis is based on the biochemistry of the amyloid fibril. We determined the NH2‐terminal sequence of an amyloid fibril and found it to be of the xI immunoglobulin subgroup. No structural alterations were detected to account for the conversion of the light‐chain fragment to an amyloid fibril. Antiserum produced to the fibril protein did not react in immunodiffusion with purified LEP or MAG antigens, which are xI proteins. This antiserum may be directed to antigenic sites unique to the immunizing protein and is unable to recognize homologous proteins, rendering it unsuitable for immunochemical identification of amyloid deposits of light‐chain origin. PAG represents the 10th reported variable xI amyloid fibril protein subjeeted to partial sequence analysis. Antisera that recognize antigenic determinants present in all members of an immunoglobulin subgroup need further development.

Original languageEnglish (US)
Pages (from-to)245-250
Number of pages6
JournalScandinavian Journal of Immunology
Volume22
Issue number3
DOIs
StatePublished - Jan 1 1985

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Amyloidogenic Proteins
Amyloid
Immune Sera
Immunoglobulins
Proteins
Light
Immunodiffusion
Amyloid Plaques
Amyloidosis
Biochemistry
Sequence Analysis
Epitopes
Antigens

ASJC Scopus subject areas

  • Immunology

Cite this

Isolation and Characterization of a x Amyloid Fibril Protein. / Gertz, Morie; SKINNER, M.; COHEN, A. S.; CONNORS, L. H.; KYLE, R. A.

In: Scandinavian Journal of Immunology, Vol. 22, No. 3, 01.01.1985, p. 245-250.

Research output: Contribution to journalArticle

Gertz, Morie ; SKINNER, M. ; COHEN, A. S. ; CONNORS, L. H. ; KYLE, R. A. / Isolation and Characterization of a x Amyloid Fibril Protein. In: Scandinavian Journal of Immunology. 1985 ; Vol. 22, No. 3. pp. 245-250.
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