Isolation and characterization of a 1 Mb region of 5q23.3-q31.2 surrounding the human lysyl oxidase gene

Terence P. McAlinden; David I. Smith; Scott E. Smith; Stephen A. Krawetz

doi:10.1016/S0022-2828(95)92235-0

Isolation and characterization of a 1 Mb region of 5q23.3-q31.2 surrounding the human lysyl oxidase gene

Terence P. McAlinden, David I. Smith, Scott E. Smith, Stephen A. Krawetz

Laboratory Medicine and Pathology

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Lysyl oxidase (EC 1.4.3.13) plays a pivotal role in the maintenance of tissue integrity in both the normal and pathological states. It is a member of a newly discovered gene family that exhibits a complex mode of regulation. To date the resources necessary to begin to address its regulation have not been assembled. In part, this reflects the instability of this region of the genome when cloned into cosmid vectors. The paucity of long range restriction endonuclease sites suitable for mapping this region of the genome has further hampered progress. To begin to address this issue 2 YAC clones of 920 kb and 245 kb that contain the human lysyl oxidase gene were isolated. Long range physical mapping revealed that the 245 kb clone was centrally located within the 920 kb clone. The corresponding map of this region is congruent with that observed in the human genome. Thus, these YACs faithfully represent this region of the human genome. The results of our cloning and mapping studies described in this communication should accelerate the advance of our understanding of this new connective tissue gene family.

Original language	English (US)
Pages (from-to)	2409-2413
Number of pages	5
Journal	Journal of Molecular and Cellular Cardiology
Volume	27
Issue number	10
DOIs	https://doi.org/10.1016/S0022-2828(95)92235-0
State	Published - Oct 1995

Keywords

Chromosome
Connective tissue
Human
Lysyl oxidase
Map
YAC

ASJC Scopus subject areas

Molecular Biology
Cardiology and Cardiovascular Medicine

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10.1016/S0022-2828(95)92235-0

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title = "Isolation and characterization of a 1 Mb region of 5q23.3-q31.2 surrounding the human lysyl oxidase gene",

abstract = "Lysyl oxidase (EC 1.4.3.13) plays a pivotal role in the maintenance of tissue integrity in both the normal and pathological states. It is a member of a newly discovered gene family that exhibits a complex mode of regulation. To date the resources necessary to begin to address its regulation have not been assembled. In part, this reflects the instability of this region of the genome when cloned into cosmid vectors. The paucity of long range restriction endonuclease sites suitable for mapping this region of the genome has further hampered progress. To begin to address this issue 2 YAC clones of 920 kb and 245 kb that contain the human lysyl oxidase gene were isolated. Long range physical mapping revealed that the 245 kb clone was centrally located within the 920 kb clone. The corresponding map of this region is congruent with that observed in the human genome. Thus, these YACs faithfully represent this region of the human genome. The results of our cloning and mapping studies described in this communication should accelerate the advance of our understanding of this new connective tissue gene family.",

keywords = "Chromosome, Connective tissue, Human, Lysyl oxidase, Map, YAC",

author = "McAlinden, {Terence P.} and Smith, {David I.} and Smith, {Scott E.} and Krawetz, {Stephen A.}",

note = "Funding Information: The authors thank Ms Dawn Cochrane for the typing of this manuscript. T. M. would like to thank D. Svinarich for his assistance with FIGE. This work was supported by Grant-in-aid 91008530 and 43GS945 to SAK from the American Heart Association (AHA) National Center and the AHA MI respectively and an AHA post-doctoral fellowship to TPM. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.",

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AU - Krawetz, Stephen A.

N1 - Funding Information: The authors thank Ms Dawn Cochrane for the typing of this manuscript. T. M. would like to thank D. Svinarich for his assistance with FIGE. This work was supported by Grant-in-aid 91008530 and 43GS945 to SAK from the American Heart Association (AHA) National Center and the AHA MI respectively and an AHA post-doctoral fellowship to TPM. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.

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N2 - Lysyl oxidase (EC 1.4.3.13) plays a pivotal role in the maintenance of tissue integrity in both the normal and pathological states. It is a member of a newly discovered gene family that exhibits a complex mode of regulation. To date the resources necessary to begin to address its regulation have not been assembled. In part, this reflects the instability of this region of the genome when cloned into cosmid vectors. The paucity of long range restriction endonuclease sites suitable for mapping this region of the genome has further hampered progress. To begin to address this issue 2 YAC clones of 920 kb and 245 kb that contain the human lysyl oxidase gene were isolated. Long range physical mapping revealed that the 245 kb clone was centrally located within the 920 kb clone. The corresponding map of this region is congruent with that observed in the human genome. Thus, these YACs faithfully represent this region of the human genome. The results of our cloning and mapping studies described in this communication should accelerate the advance of our understanding of this new connective tissue gene family.

AB - Lysyl oxidase (EC 1.4.3.13) plays a pivotal role in the maintenance of tissue integrity in both the normal and pathological states. It is a member of a newly discovered gene family that exhibits a complex mode of regulation. To date the resources necessary to begin to address its regulation have not been assembled. In part, this reflects the instability of this region of the genome when cloned into cosmid vectors. The paucity of long range restriction endonuclease sites suitable for mapping this region of the genome has further hampered progress. To begin to address this issue 2 YAC clones of 920 kb and 245 kb that contain the human lysyl oxidase gene were isolated. Long range physical mapping revealed that the 245 kb clone was centrally located within the 920 kb clone. The corresponding map of this region is congruent with that observed in the human genome. Thus, these YACs faithfully represent this region of the human genome. The results of our cloning and mapping studies described in this communication should accelerate the advance of our understanding of this new connective tissue gene family.

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Isolation and characterization of a 1 Mb region of 5q23.3-q31.2 surrounding the human lysyl oxidase gene

Abstract

Keywords

ASJC Scopus subject areas

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