Isolation and analysis of the proteins of plant nuclei from two stages of differentiation of Phaseolus vulgaris

Thomas C. Spelsberg, Igor V. Sarkissian

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The proteins of plant nuclei from Phaseolus vulgaris were separated and analyzed by polyacrylamide gel electrophoresis and amino acid analysis. Comparative studies of some protein fractions revealed quantitative and qualitative differences between the undifferentiated hook and the more differentiated lower hypocotyl of bean. While the histone fractions displayed similar electrophoretic patterns, the acidic protein fractions varied accordingly to the stage of development. Various protein fractions from the same tissue differed markedly in amino acid composition; there was little variation in similar fractions from the two stages of differentiation.

Original languageEnglish (US)
Pages (from-to)1385-1391
Number of pages7
JournalPhytochemistry
Volume9
Issue number7
DOIs
StatePublished - 1970
Externally publishedYes

Fingerprint

Plant Proteins
Phaseolus
plant proteins
Phaseolus vulgaris
Amino Acids
Hypocotyl
Proteins
proteins
Hooks
Electrophoresis
amino acid composition
histones
Histones
hypocotyls
polyacrylamide gel electrophoresis
Polyacrylamide Gel Electrophoresis
beans
Tissue
amino acids
Chemical analysis

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry
  • Molecular Biology
  • Organic Chemistry
  • Drug Discovery

Cite this

Isolation and analysis of the proteins of plant nuclei from two stages of differentiation of Phaseolus vulgaris. / Spelsberg, Thomas C.; Sarkissian, Igor V.

In: Phytochemistry, Vol. 9, No. 7, 1970, p. 1385-1391.

Research output: Contribution to journalArticle

Spelsberg, Thomas C. ; Sarkissian, Igor V. / Isolation and analysis of the proteins of plant nuclei from two stages of differentiation of Phaseolus vulgaris. In: Phytochemistry. 1970 ; Vol. 9, No. 7. pp. 1385-1391.
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