Ischemia-reperfusion injury causes oxidative stress and apoptosis of Schwann cell in acute and chronic experimental diabetic neuropathy

Yanping Wang, Ann M. Schmeichel, Haruyasu Iida, James D. Schmelzer, Phillip Anson Low

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Abstract

Mild ischemia-reperfusion (IR) injury to diabetic peripheral nerve is known to cause severe ischemic fiber degeneration. Little information is available on its effects on Schwann cell (SC). In this study, we evaluated oxidative stress and apoptosis of SC following mild IR, using immunohistochemistry in streptozotocin (STZ)-induced diabetic rats. Twenty-six rats were divided into four groups according to the duration of diabetes: 1-month STZ-induced diabetic group (n = 7) and age-matched control group (n = 7); 4-month STZ-induced diabetic group (n = 6) and age-matched control group (n = 6). Using our established IR model of 3 h of ischemia followed by 7 days of reperfusion, sciatic and tibial nerves were harvested and labeled with 8- hydroxydeoxyguanosine (8-OHdG; oxidative stress marker), caspase-3 (apoptotic executor), and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) activity (apoptotic indicator). Marked positive staining with 8-OHdG, caspase-3, and TUNEL were found in diabetic ischemic nerves (right side) following IR in both 1-month and 4-month groups. Only mild positive staining or no staining was seen in the nonischemic side (left side) of diabetic and age-matched control groups. Co-labeling with S-100 confirmed that the cells labeled with 8-OHdG, caspaseS, and TUNEL were SC. SC was susceptible to oxidative injury and apoptosis in experimental diabetic neuropathy when subjected to mild IR injury.

Original languageEnglish (US)
Pages (from-to)1513-1520
Number of pages8
JournalAntioxidants and Redox Signaling
Volume7
Issue number11-12
DOIs
StatePublished - Nov 2005

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Oxidative stress
Diabetic Neuropathies
Schwann Cells
Reperfusion Injury
Labeling
Reperfusion
Oxidative Stress
Ischemia
Streptozocin
Cells
Apoptosis
Research Design
Staining and Labeling
Transferases
Caspase 3
Control Groups
Rats
Tibial Nerve
DNA Nucleotidylexotransferase
Caspase 8

ASJC Scopus subject areas

  • Biochemistry

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Ischemia-reperfusion injury causes oxidative stress and apoptosis of Schwann cell in acute and chronic experimental diabetic neuropathy. / Wang, Yanping; Schmeichel, Ann M.; Iida, Haruyasu; Schmelzer, James D.; Low, Phillip Anson.

In: Antioxidants and Redox Signaling, Vol. 7, No. 11-12, 11.2005, p. 1513-1520.

Research output: Contribution to journalArticle

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