Is persistent polyclonal B lymphocytosis caused by Epstein-Barr virus? A study with polymerase chain reaction and in situ hybridization

K. C. Chow, Thomas Elmer Witzig, T. E. Witzig, C. Y. Li

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Previous reports of patients with persistent polyclonal B lymphocytosis have found associations with female sex, cigarette smoking, HLA-DR7 phenotype, and moderate elevation of peripheral blood polyclonal B lymphocytes. The presence of binucleated cells and atypical lymphocytes in the peripheral blood of these patients was highly suggestive of a viral infection, such as with the Epstein-Barr virus. We report a 47-year-old asymptomatic woman who was incidentally found to have persistent polyclonal B lymphocytosis and serum IgG against virus capsid antigen (VCA) and Epstein-Barr virus (EBV)-associated nuclear antigen (EBNA) of EBV. The presence of EBV was investigated in the peripheral blood lymphocytes from this patient by in situ hybridization and polymerase chain reaction methods. EBV DNA was demonstrated in the lymphocyte fraction by polymerase chain reaction, and it was further located in lymphoid cells by in situ hybridization. These results indicate that persistent polyclonal B lymphocytosis is strongly associated with EBV.

Original languageEnglish (US)
Pages (from-to)270-275
Number of pages6
JournalAmerican Journal of Hematology
Volume41
Issue number4
DOIs
StatePublished - 1992

Fingerprint

Lymphocytosis
Human Herpesvirus 4
In Situ Hybridization
Polymerase Chain Reaction
Lymphocytes
HLA-DR7 Antigen
Epstein-Barr Virus Nuclear Antigens
Capsid
Virus Diseases
B-Lymphocytes
Immunoglobulin G
Smoking
Viruses
Phenotype
Antigens
DNA
Serum

Keywords

  • EBNA
  • EBV
  • smoking

ASJC Scopus subject areas

  • Hematology

Cite this

Is persistent polyclonal B lymphocytosis caused by Epstein-Barr virus? A study with polymerase chain reaction and in situ hybridization. / Chow, K. C.; Witzig, Thomas Elmer; Witzig, T. E.; Li, C. Y.

In: American Journal of Hematology, Vol. 41, No. 4, 1992, p. 270-275.

Research output: Contribution to journalArticle

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