Iron regulatory protein-1 protects against mitoferrin-1-deficient porphyria

Jacky Chung, Sheila A. Anderson, Babette Gwynn, Kathryn M. Deck, Michael J. Chen, Nathaniel B. Langer, George C. Shaw, Nicholas C. Huston, Leah F. Boyer, Sumon Datta, Prasad N. Paradkar, Liangtao Li, Zong Wei, Amy J. Lambert, Kenneth Sahr, Johannes G. Wittig, Wen Chen, Wange Lu, Bruno Galy, Thorsten M. SchlaegerMatthias W. Hentze, Diane M. Ward, Jerry Kaplan, Richard S. Eisenstein, Luanne L. Peters, Barry H. Paw

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Mitochondrial iron is essential for the biosynthesis of heme and iron-sulfur ([Fe-S]) clusters in mammalian cells. In developing erythrocytes, iron is imported into the mitochondria by MFRN1 (mitoferrin-1, SLC25A37). Although loss of MFRN1 in zebrafish and mice leads to profound anemia, mutant animals showed no overt signs of porphyria, suggesting that mitochondrial iron deficiency does not result in an accumulation of protoporphyrins. Here, we developed a gene trap model to provide in vitro and in vivo evidence that iron regulatory protein-1 (IRP1) inhibits protoporphyrin accumulation. Mfrn1 +/gt; Irp1-/- erythroid cells exhibit a significant increase in protoporphyrin levels. IRP1 attenuates protoporphyrin biosynthesis by binding to the 5′-iron response element (IRE) of alas2 mRNA, inhibiting its translation. Ectopic expression of alas2 harboring a mutant IRE, preventing IRP1 binding, in Mfrn1gt/gt cells mimics Irp1 deficiency. Together, our data support a model whereby impaired mitochondrial [Fe-S] cluster biogenesis in Mfrn1gt/gt cells results in elevated IRP1 RNA-binding that attenuates ALAS2 mRNA translation and protoporphyrin accumulation.

Original languageEnglish (US)
Pages (from-to)7835-7843
Number of pages9
JournalJournal of Biological Chemistry
Volume289
Issue number11
DOIs
StatePublished - Mar 14 2014

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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