Ion and solute transport by Prestin in Drosophila and Anopheles

Taku Hirata, Anna Czapar, Lauren Brin, Alyona Haritonova, Daniel P. Bondeson, Paul Linser, Pablo Cabrero, James Thompson, Julian A T Dow, Michael F Romero

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The gut and Malpighian tubules of insects are the primary sites of active solute and water transport for controlling hemolymph and urine composition, pH, and osmolarity. These processes depend on ATPase (pumps), channels and solute carriers (Slc proteins). Maturation of genomic databases enables us to identify the putative molecular players for these processes. Anion transporters of the Slc4 family, AE1 and NDAE1, have been reported as HCO3- transporters, but are only part of the story. Here we report Dipteran (Drosophila melanogaster (d) and Anopheles gambiae (Ag)) anion exchangers, belonging to the Slc26 family, which are multi-functional anion exchangers. One Drosophila and two Ag homologues of mammalian Slc26a5 (Prestin) and Slc26a6 (aka, PAT1, CFEX) were identified and designated dPrestin, AgPrestinA and AgPrestinB. dPrestin and AgPrestinB show electrogenic anion exchange (Cl -/nHCO3-, Cl-/SO42- and Cl -/oxalate 2-) in an oocyte expression system. Since these transporters are the only Dipteran Slc26 proteins whose transport is similar to mammalian Slc26a6, we submit that Dipteran Prestin are functional and even molecular orthologues of mammalian Slc26a6. OSR1 kinase increases dPrestin ion transport, implying another set of physiological processes controlled by WNK/SPAK signaling in epithelia. All of these mRNAs are highly expressed in the gut and Malpighian tubules. Dipteran Prestin proteins appear suited for central roles in bicarbonate, sulfate and oxalate metabolism including generating the high pH conditions measured in the Dipteran midgut lumen. Finally, we present and discuss Drosophila genetic models that integrate these processes.

Original languageEnglish (US)
Pages (from-to)563-569
Number of pages7
JournalJournal of Insect Physiology
Volume58
Issue number4
DOIs
StatePublished - Apr 2012

Fingerprint

Anopheles
Ion Transport
ion transport
Drosophila
Anions
Malpighian Tubules
Anopheles gambiae
Oxalates
transporters
antiporters
Malpighian tubules
oxalates
solutes
Physiological Phenomena
digestive system
Hemolymph
Genetic Models
Protein Transport
Bicarbonates
Drosophila melanogaster

Keywords

  • Cl transport
  • Gut
  • Malpighian tubules
  • Prestin
  • Slc26

ASJC Scopus subject areas

  • Insect Science
  • Physiology

Cite this

Hirata, T., Czapar, A., Brin, L., Haritonova, A., Bondeson, D. P., Linser, P., ... Romero, M. F. (2012). Ion and solute transport by Prestin in Drosophila and Anopheles. Journal of Insect Physiology, 58(4), 563-569. https://doi.org/10.1016/j.jinsphys.2012.01.009

Ion and solute transport by Prestin in Drosophila and Anopheles. / Hirata, Taku; Czapar, Anna; Brin, Lauren; Haritonova, Alyona; Bondeson, Daniel P.; Linser, Paul; Cabrero, Pablo; Thompson, James; Dow, Julian A T; Romero, Michael F.

In: Journal of Insect Physiology, Vol. 58, No. 4, 04.2012, p. 563-569.

Research output: Contribution to journalArticle

Hirata, T, Czapar, A, Brin, L, Haritonova, A, Bondeson, DP, Linser, P, Cabrero, P, Thompson, J, Dow, JAT & Romero, MF 2012, 'Ion and solute transport by Prestin in Drosophila and Anopheles', Journal of Insect Physiology, vol. 58, no. 4, pp. 563-569. https://doi.org/10.1016/j.jinsphys.2012.01.009
Hirata T, Czapar A, Brin L, Haritonova A, Bondeson DP, Linser P et al. Ion and solute transport by Prestin in Drosophila and Anopheles. Journal of Insect Physiology. 2012 Apr;58(4):563-569. https://doi.org/10.1016/j.jinsphys.2012.01.009
Hirata, Taku ; Czapar, Anna ; Brin, Lauren ; Haritonova, Alyona ; Bondeson, Daniel P. ; Linser, Paul ; Cabrero, Pablo ; Thompson, James ; Dow, Julian A T ; Romero, Michael F. / Ion and solute transport by Prestin in Drosophila and Anopheles. In: Journal of Insect Physiology. 2012 ; Vol. 58, No. 4. pp. 563-569.
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