Involvement of RNA helicases p68 and p72 in colon cancer

Sook Shin, Kari L. Rossow, Joseph P. Grande, Ralf Janknecht

Research output: Contribution to journalArticle

109 Scopus citations

Abstract

The homologous proteins p68 and p72 are members of the DEAD box family of RNA helicases. Here, we show that expression of both of these helicases strongly increases during the polyp→adenoma→adenocarcinoma transition in the colon. Furthermore, p68 and p72 form complexes with β-catenin and promote the ability of β-catenin to activate gene transcription. Conversely, simultaneous knockdown of p68 and p72 leads to reduced expression of the β-catenin-regulated genes, c-Myc, cyclin D1, c-jun, and fra-1, all of which are proto-oncogenes. Moreover, transcription of the cell cycle inhibitor p21WAF1/CIP1, whose expression is suppressed by c-Myc, is enhanced on p68/p72 knockdown. Thus, p68/p72 may contribute to colon cancer formation by directly upregulating proto-oncogenes and indirectly by down-regulating the growth suppressor p21WAF1/CIP1. Accordingly, knockdown of p68 and p72 in colon cancer cells inhibits their proliferation and diminishes their ability to form tumors in vivo. Altogether, these results suggest that p68/p72 overexpression is not only a potential marker of colon cancer but is also causally linked to this disease. Therefore, p68 and p72 may be novel targets in the combat against colon cancer.

Original languageEnglish (US)
Pages (from-to)7572-7578
Number of pages7
JournalCancer research
Volume67
Issue number16
DOIs
StatePublished - Aug 15 2007

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ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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