Invited review: Significance of spatial and temporal heterogeneity of calcium transients in smooth muscle

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Abstract

The multiplicity of mechanisms involved in regulation of intracellular Ca2+ concentration ([Ca2+]i) in smooth muscle results in both intra- and intercellular heterogeneities in [Ca2+]i. Heterogeneity in [Ca2+]i regulation is reflected by the presence of spontaneous, localized [Ca2+]i transients (Ca2+ sparks) representing Ca2+ release through ryanodine receptor (RyR) channels. Ca2+ sparks display variable spatial Ca2+ distributions with every occurrence within and across cellular regions. Individual sparks are often grouped, and fusion of sparks produces large local elevations in [Ca2+]i that occasionally trigger propagating [Ca2+]i waves. Ca2+ sparks may modulate membrane potential and thus smooth muscle contractility. Sparks may also be the target of other regulatory factors in smooth muscle. Agonists induce propagating [Ca2+]i oscillations that originate from foci with high spark incidence and also represent Ca2+ release through RyR channels. With increasing agonist concentration, the peak of regional [Ca2+]i oscillations remains relatively constant, whereas both frequency and propagation velocity increase. In contrast, the global cellular response appears as a concentration-dependent increase in peak as well as mean cellular [Ca2+]i, representing a spatial and temporal integration of the oscillations. The significance of agonist-induced [Ca2+]i oscillations lies in the establishment of a global [Ca2+]i level for slower Ca2+ -dependent physiological processes.

Original languageEnglish (US)
Pages (from-to)488-496
Number of pages9
JournalJournal of Applied Physiology
Volume91
Issue number1
StatePublished - 2001

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Smooth Muscle
Ryanodine Receptor Calcium Release Channel
Calcium
Physiological Phenomena
Membrane Potentials
Incidence

Keywords

  • Acetylcholine
  • Calcium oscillations
  • Calcium sparks
  • Confocal microscopy
  • Fluorescence
  • Ryanodine receptor
  • Sarcoplasmic reticulum

ASJC Scopus subject areas

  • Endocrinology
  • Physiology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

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title = "Invited review: Significance of spatial and temporal heterogeneity of calcium transients in smooth muscle",
abstract = "The multiplicity of mechanisms involved in regulation of intracellular Ca2+ concentration ([Ca2+]i) in smooth muscle results in both intra- and intercellular heterogeneities in [Ca2+]i. Heterogeneity in [Ca2+]i regulation is reflected by the presence of spontaneous, localized [Ca2+]i transients (Ca2+ sparks) representing Ca2+ release through ryanodine receptor (RyR) channels. Ca2+ sparks display variable spatial Ca2+ distributions with every occurrence within and across cellular regions. Individual sparks are often grouped, and fusion of sparks produces large local elevations in [Ca2+]i that occasionally trigger propagating [Ca2+]i waves. Ca2+ sparks may modulate membrane potential and thus smooth muscle contractility. Sparks may also be the target of other regulatory factors in smooth muscle. Agonists induce propagating [Ca2+]i oscillations that originate from foci with high spark incidence and also represent Ca2+ release through RyR channels. With increasing agonist concentration, the peak of regional [Ca2+]i oscillations remains relatively constant, whereas both frequency and propagation velocity increase. In contrast, the global cellular response appears as a concentration-dependent increase in peak as well as mean cellular [Ca2+]i, representing a spatial and temporal integration of the oscillations. The significance of agonist-induced [Ca2+]i oscillations lies in the establishment of a global [Ca2+]i level for slower Ca2+ -dependent physiological processes.",
keywords = "Acetylcholine, Calcium oscillations, Calcium sparks, Confocal microscopy, Fluorescence, Ryanodine receptor, Sarcoplasmic reticulum",
author = "Pabelick, {Christina M} and Sieck, {Gary C} and Y.s. Prakash",
year = "2001",
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AU - Pabelick, Christina M

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AU - Prakash, Y.s.

PY - 2001

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N2 - The multiplicity of mechanisms involved in regulation of intracellular Ca2+ concentration ([Ca2+]i) in smooth muscle results in both intra- and intercellular heterogeneities in [Ca2+]i. Heterogeneity in [Ca2+]i regulation is reflected by the presence of spontaneous, localized [Ca2+]i transients (Ca2+ sparks) representing Ca2+ release through ryanodine receptor (RyR) channels. Ca2+ sparks display variable spatial Ca2+ distributions with every occurrence within and across cellular regions. Individual sparks are often grouped, and fusion of sparks produces large local elevations in [Ca2+]i that occasionally trigger propagating [Ca2+]i waves. Ca2+ sparks may modulate membrane potential and thus smooth muscle contractility. Sparks may also be the target of other regulatory factors in smooth muscle. Agonists induce propagating [Ca2+]i oscillations that originate from foci with high spark incidence and also represent Ca2+ release through RyR channels. With increasing agonist concentration, the peak of regional [Ca2+]i oscillations remains relatively constant, whereas both frequency and propagation velocity increase. In contrast, the global cellular response appears as a concentration-dependent increase in peak as well as mean cellular [Ca2+]i, representing a spatial and temporal integration of the oscillations. The significance of agonist-induced [Ca2+]i oscillations lies in the establishment of a global [Ca2+]i level for slower Ca2+ -dependent physiological processes.

AB - The multiplicity of mechanisms involved in regulation of intracellular Ca2+ concentration ([Ca2+]i) in smooth muscle results in both intra- and intercellular heterogeneities in [Ca2+]i. Heterogeneity in [Ca2+]i regulation is reflected by the presence of spontaneous, localized [Ca2+]i transients (Ca2+ sparks) representing Ca2+ release through ryanodine receptor (RyR) channels. Ca2+ sparks display variable spatial Ca2+ distributions with every occurrence within and across cellular regions. Individual sparks are often grouped, and fusion of sparks produces large local elevations in [Ca2+]i that occasionally trigger propagating [Ca2+]i waves. Ca2+ sparks may modulate membrane potential and thus smooth muscle contractility. Sparks may also be the target of other regulatory factors in smooth muscle. Agonists induce propagating [Ca2+]i oscillations that originate from foci with high spark incidence and also represent Ca2+ release through RyR channels. With increasing agonist concentration, the peak of regional [Ca2+]i oscillations remains relatively constant, whereas both frequency and propagation velocity increase. In contrast, the global cellular response appears as a concentration-dependent increase in peak as well as mean cellular [Ca2+]i, representing a spatial and temporal integration of the oscillations. The significance of agonist-induced [Ca2+]i oscillations lies in the establishment of a global [Ca2+]i level for slower Ca2+ -dependent physiological processes.

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