Investigation of continuous cyclodextrin glucanotransferase production by the alginate-immobilized cells of alkalophilic Bacillus sp. in an airlift reactor

Adinarayana Kunamneni; Taadimalla Prabhakar; Bezawada Jyothi; Poluri Ellaiah

doi:10.1016/j.enzmictec.2006.10.031

Investigation of continuous cyclodextrin glucanotransferase production by the alginate-immobilized cells of alkalophilic Bacillus sp. in an airlift reactor

Adinarayana Kunamneni, Taadimalla Prabhakar, Bezawada Jyothi, Poluri Ellaiah

Infectious Diseases

Research output: Contribution to journal › Article › peer-review

Abstract

Cyclodextrin glucanotransferase (CGTase) production by free and calcium alginate-immobilized cells was investigated in an airlift reactor. The average volumetric productivity with continuous fermentation (13.65 U ml^-1 h^-1) was found to be greater than the free cells (6.89 U ml^-1 h^-1). The total CGTase enzyme produced with continuous fermentation was found to be about 98% greater than with that of free cells. The immobilized Bacillus particles showed a half-life of 18 days during continuous fermentation under airlift conditions.

Original language	English (US)
Pages (from-to)	1538-1542
Number of pages	5
Journal	Enzyme and Microbial Technology
Volume	40
Issue number	6
DOIs	https://doi.org/10.1016/j.enzmictec.2006.10.031
State	Published - May 2 2007

Keywords

Airlift reactor
Alkalophilic Bacillus sp.
Continuous fermentation
Cyclodextrin glucanotransferase
Immobilized cells
Volumetric productivity

ASJC Scopus subject areas

Biotechnology
Bioengineering
Biochemistry
Applied Microbiology and Biotechnology

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10.1016/j.enzmictec.2006.10.031

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Investigation of continuous cyclodextrin glucanotransferase production by the alginate-immobilized cells of alkalophilic Bacillus sp. in an airlift reactor. / Kunamneni, Adinarayana; Prabhakar, Taadimalla; Jyothi, Bezawada et al.
In: Enzyme and Microbial Technology, Vol. 40, No. 6, 02.05.2007, p. 1538-1542.

Research output: Contribution to journal › Article › peer-review

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title = "Investigation of continuous cyclodextrin glucanotransferase production by the alginate-immobilized cells of alkalophilic Bacillus sp. in an airlift reactor",

abstract = "Cyclodextrin glucanotransferase (CGTase) production by free and calcium alginate-immobilized cells was investigated in an airlift reactor. The average volumetric productivity with continuous fermentation (13.65 U ml-1 h-1) was found to be greater than the free cells (6.89 U ml-1 h-1). The total CGTase enzyme produced with continuous fermentation was found to be about 98% greater than with that of free cells. The immobilized Bacillus particles showed a half-life of 18 days during continuous fermentation under airlift conditions.",

keywords = "Airlift reactor, Alkalophilic Bacillus sp., Continuous fermentation, Cyclodextrin glucanotransferase, Immobilized cells, Volumetric productivity",

author = "Adinarayana Kunamneni and Taadimalla Prabhakar and Bezawada Jyothi and Poluri Ellaiah",

note = "Funding Information: The authors are thankful to Andhra University, Visakhapatnam, for providing the financial support to carry out the research work.",

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AU - Prabhakar, Taadimalla

AU - Jyothi, Bezawada

AU - Ellaiah, Poluri

N1 - Funding Information: The authors are thankful to Andhra University, Visakhapatnam, for providing the financial support to carry out the research work.

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N2 - Cyclodextrin glucanotransferase (CGTase) production by free and calcium alginate-immobilized cells was investigated in an airlift reactor. The average volumetric productivity with continuous fermentation (13.65 U ml-1 h-1) was found to be greater than the free cells (6.89 U ml-1 h-1). The total CGTase enzyme produced with continuous fermentation was found to be about 98% greater than with that of free cells. The immobilized Bacillus particles showed a half-life of 18 days during continuous fermentation under airlift conditions.

AB - Cyclodextrin glucanotransferase (CGTase) production by free and calcium alginate-immobilized cells was investigated in an airlift reactor. The average volumetric productivity with continuous fermentation (13.65 U ml-1 h-1) was found to be greater than the free cells (6.89 U ml-1 h-1). The total CGTase enzyme produced with continuous fermentation was found to be about 98% greater than with that of free cells. The immobilized Bacillus particles showed a half-life of 18 days during continuous fermentation under airlift conditions.

KW - Airlift reactor

KW - Alkalophilic Bacillus sp.

KW - Continuous fermentation

KW - Cyclodextrin glucanotransferase

KW - Immobilized cells

KW - Volumetric productivity

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Investigation of continuous cyclodextrin glucanotransferase production by the alginate-immobilized cells of alkalophilic Bacillus sp. in an airlift reactor

Abstract

Keywords

ASJC Scopus subject areas

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