Intravitreal alemtuzumab penetrates full-thickness retina in rabbit eyes

Noha S. Ekdawi; Jose S. Pulido; Sujit Itty; Ronald J. Marler; David C. Herman; Paul Hardwig; Brian G. Mohney; Tibor Valyi-Nagy; Deepak Shukla

doi:10.1097/IAE.0b013e3181b901df

Intravitreal alemtuzumab penetrates full-thickness retina in rabbit eyes

Noha S. Ekdawi, Jose S. Pulido, Sujit Itty, Ronald J. Marler, David C. Herman, Paul Hardwig, Brian G. Mohney, Tibor Valyi-Nagy, Deepak Shukla

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Purpose: The purpose of this study was to assess whether alemtuzamab, a large antibody of 150 kDa, would be able to penetrate through the full-thickness retina of Dutch-belted rabbits. Methods: Four Dutch-belted rabbits had intravitreal injections of alemtuzumab (1.5 mg in 0.05 mL). One rabbit each was killed at Day 1, Day 8, Day 15, and Day 29. The eyes were examined under frozen section and graded by immunostaining techniques for the degree of penetration of alemtuzumab into the retina. The degree of retinal staining was graded from 0 (no stain) to 4+ (marked stain). Results: All study eyes showed antibody staining of the full-thickness retina as follows: 4+ at Day 1, 4+ at Day 8, 3+ at Day 15, and 2+ at Day 29. Conclusion: A 1.5-mg intravitreal dose of alemtuzumab was able to penetrate full-thickness retina throughout the full 29-day course of the study. Retinal toxicity studies are required before clinical use.

Original language	English (US)
Pages (from-to)	1532-1534
Number of pages	3
Journal	Retina
Volume	29
Issue number	10
DOIs	https://doi.org/10.1097/IAE.0b013e3181b901df
State	Published - Nov 2009

ASJC Scopus subject areas

Ophthalmology

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title = "Intravitreal alemtuzumab penetrates full-thickness retina in rabbit eyes",

abstract = "Purpose: The purpose of this study was to assess whether alemtuzamab, a large antibody of 150 kDa, would be able to penetrate through the full-thickness retina of Dutch-belted rabbits. Methods: Four Dutch-belted rabbits had intravitreal injections of alemtuzumab (1.5 mg in 0.05 mL). One rabbit each was killed at Day 1, Day 8, Day 15, and Day 29. The eyes were examined under frozen section and graded by immunostaining techniques for the degree of penetration of alemtuzumab into the retina. The degree of retinal staining was graded from 0 (no stain) to 4+ (marked stain). Results: All study eyes showed antibody staining of the full-thickness retina as follows: 4+ at Day 1, 4+ at Day 8, 3+ at Day 15, and 2+ at Day 29. Conclusion: A 1.5-mg intravitreal dose of alemtuzumab was able to penetrate full-thickness retina throughout the full 29-day course of the study. Retinal toxicity studies are required before clinical use.",

author = "Ekdawi, {Noha S.} and Pulido, {Jose S.} and Sujit Itty and Marler, {Ronald J.} and Herman, {David C.} and Paul Hardwig and Mohney, {Brian G.} and Tibor Valyi-Nagy and Deepak Shukla",

year = "2009",

month = nov,

doi = "10.1097/IAE.0b013e3181b901df",

language = "English (US)",

volume = "29",

pages = "1532--1534",

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T1 - Intravitreal alemtuzumab penetrates full-thickness retina in rabbit eyes

AU - Ekdawi, Noha S.

AU - Pulido, Jose S.

AU - Itty, Sujit

AU - Marler, Ronald J.

AU - Herman, David C.

AU - Hardwig, Paul

AU - Mohney, Brian G.

AU - Valyi-Nagy, Tibor

AU - Shukla, Deepak

PY - 2009/11

Y1 - 2009/11

N2 - Purpose: The purpose of this study was to assess whether alemtuzamab, a large antibody of 150 kDa, would be able to penetrate through the full-thickness retina of Dutch-belted rabbits. Methods: Four Dutch-belted rabbits had intravitreal injections of alemtuzumab (1.5 mg in 0.05 mL). One rabbit each was killed at Day 1, Day 8, Day 15, and Day 29. The eyes were examined under frozen section and graded by immunostaining techniques for the degree of penetration of alemtuzumab into the retina. The degree of retinal staining was graded from 0 (no stain) to 4+ (marked stain). Results: All study eyes showed antibody staining of the full-thickness retina as follows: 4+ at Day 1, 4+ at Day 8, 3+ at Day 15, and 2+ at Day 29. Conclusion: A 1.5-mg intravitreal dose of alemtuzumab was able to penetrate full-thickness retina throughout the full 29-day course of the study. Retinal toxicity studies are required before clinical use.

AB - Purpose: The purpose of this study was to assess whether alemtuzamab, a large antibody of 150 kDa, would be able to penetrate through the full-thickness retina of Dutch-belted rabbits. Methods: Four Dutch-belted rabbits had intravitreal injections of alemtuzumab (1.5 mg in 0.05 mL). One rabbit each was killed at Day 1, Day 8, Day 15, and Day 29. The eyes were examined under frozen section and graded by immunostaining techniques for the degree of penetration of alemtuzumab into the retina. The degree of retinal staining was graded from 0 (no stain) to 4+ (marked stain). Results: All study eyes showed antibody staining of the full-thickness retina as follows: 4+ at Day 1, 4+ at Day 8, 3+ at Day 15, and 2+ at Day 29. Conclusion: A 1.5-mg intravitreal dose of alemtuzumab was able to penetrate full-thickness retina throughout the full 29-day course of the study. Retinal toxicity studies are required before clinical use.

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Intravitreal alemtuzumab penetrates full-thickness retina in rabbit eyes

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