Intravital imaging allows real-time characterization of tissue resident eosinophils

Andrew Chojnacki, Katarzyna Wojcik, Björn Petri, Gurpreet Aulakh, Elizabeth A. Jacobsen, William E. LeSuer, Pina Colarusso, Kamala D. Patel

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Eosinophils are core components of the immune system, yet tools are lacking to directly observe eosinophils in action in vivo. To better understand the role of tissue resident eosinophils, we used eosinophil-specific CRE (eoCRE) mice to create GFP and tdTomato reporters. We then employed intravital microscopy to examine the dynamic behaviour of eosinophils in the healthy GI tract, mesentery, liver, lymph node, skin and lung. Given the role of eosinophils in allergic airway diseases, we also examined eosinophils in the lung following ovalbumin sensitization and challenge. We were able to monitor and quantify eosinophilic behaviours including patrolling, crawling, clustering, tissue distribution and interactions with other leukocytes. Thus, these reporter mice allow eosinophils to be examined in real-time in living animals, paving the way to further understanding the roles eosinophils play in both health and disease.

Original languageEnglish (US)
Article number181
JournalCommunications Biology
Volume2
Issue number1
DOIs
StatePublished - Dec 1 2019

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • Medicine (miscellaneous)

Fingerprint Dive into the research topics of 'Intravital imaging allows real-time characterization of tissue resident eosinophils'. Together they form a unique fingerprint.

  • Cite this

    Chojnacki, A., Wojcik, K., Petri, B., Aulakh, G., Jacobsen, E. A., LeSuer, W. E., Colarusso, P., & Patel, K. D. (2019). Intravital imaging allows real-time characterization of tissue resident eosinophils. Communications Biology, 2(1), [181]. https://doi.org/10.1038/s42003-019-0425-3