Intravenous Injection of a Lentiviral Vector Encoding NY-ESO-1 Induces an Effective CTL Response

Michael J. Palmowski, Luciene Lopes, Yasuhiro Ikeda, Mariolina Salio, Vincenzo Cerundolo, Mary K. Collins

Research output: Contribution to journalArticlepeer-review

90 Scopus citations

Abstract

Lentiviral vectors can efficiently transduce a variety of nondividing cells, including APCs. We assessed the immunogenicity of a lentiviral vector encoding the melanoma Ag NY-ESO-1 in HLA-A2 transgenic mice. Direct i.v. injection of NY-ESO-1 lentivirus induced NY-ESO-1157-165-specific CD8+ cells, detected ex vivo with an A2/H-2Kb chimeric class I tetramer. These NY-ESO-1157-165-specific CD8+ cells could be expanded by boosting with an NY-ESO-1 vaccinia virus and could kill NY-ESO-1157-165 peptide-pulsed targets in vivo. Such direct lentiviral vector injection was similar in potency to the injection of in vitro-transduced dendritic cells (DC). In addition, human monocyte-derived DC transduced by the NY-ESO-1 lentivirus stimulated an NY-ESO-1 157-165-specific specific CTL clone. These data suggest that direct lentiviral transduction of DC in vivo might provide a powerful immunotherapeutic strategy.

Original languageEnglish (US)
Pages (from-to)1582-1587
Number of pages6
JournalJournal of Immunology
Volume172
Issue number3
DOIs
StatePublished - Feb 1 2004

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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