Intravenous Injection of a Lentiviral Vector Encoding NY-ESO-1 Induces an Effective CTL Response

Michael J. Palmowski, Luciene Lopes, Yasuhiro H Ikeda, Mariolina Salio, Vincenzo Cerundolo, Mary K. Collins

Research output: Contribution to journalArticle

89 Citations (Scopus)

Abstract

Lentiviral vectors can efficiently transduce a variety of nondividing cells, including APCs. We assessed the immunogenicity of a lentiviral vector encoding the melanoma Ag NY-ESO-1 in HLA-A2 transgenic mice. Direct i.v. injection of NY-ESO-1 lentivirus induced NY-ESO-1157-165-specific CD8+ cells, detected ex vivo with an A2/H-2Kb chimeric class I tetramer. These NY-ESO-1157-165-specific CD8+ cells could be expanded by boosting with an NY-ESO-1 vaccinia virus and could kill NY-ESO-1157-165 peptide-pulsed targets in vivo. Such direct lentiviral vector injection was similar in potency to the injection of in vitro-transduced dendritic cells (DC). In addition, human monocyte-derived DC transduced by the NY-ESO-1 lentivirus stimulated an NY-ESO-1 157-165-specific specific CTL clone. These data suggest that direct lentiviral transduction of DC in vivo might provide a powerful immunotherapeutic strategy.

Original languageEnglish (US)
Pages (from-to)1582-1587
Number of pages6
JournalJournal of Immunology
Volume172
Issue number3
StatePublished - Feb 1 2004
Externally publishedYes

Fingerprint

Intravenous Injections
Dendritic Cells
Lentivirus
Injections
HLA-A2 Antigen
Vaccinia virus
varespladib methyl
Transgenic Mice
Monocytes
Melanoma
Clone Cells
Peptides
In Vitro Techniques

ASJC Scopus subject areas

  • Immunology

Cite this

Palmowski, M. J., Lopes, L., Ikeda, Y. H., Salio, M., Cerundolo, V., & Collins, M. K. (2004). Intravenous Injection of a Lentiviral Vector Encoding NY-ESO-1 Induces an Effective CTL Response. Journal of Immunology, 172(3), 1582-1587.

Intravenous Injection of a Lentiviral Vector Encoding NY-ESO-1 Induces an Effective CTL Response. / Palmowski, Michael J.; Lopes, Luciene; Ikeda, Yasuhiro H; Salio, Mariolina; Cerundolo, Vincenzo; Collins, Mary K.

In: Journal of Immunology, Vol. 172, No. 3, 01.02.2004, p. 1582-1587.

Research output: Contribution to journalArticle

Palmowski, MJ, Lopes, L, Ikeda, YH, Salio, M, Cerundolo, V & Collins, MK 2004, 'Intravenous Injection of a Lentiviral Vector Encoding NY-ESO-1 Induces an Effective CTL Response', Journal of Immunology, vol. 172, no. 3, pp. 1582-1587.
Palmowski MJ, Lopes L, Ikeda YH, Salio M, Cerundolo V, Collins MK. Intravenous Injection of a Lentiviral Vector Encoding NY-ESO-1 Induces an Effective CTL Response. Journal of Immunology. 2004 Feb 1;172(3):1582-1587.
Palmowski, Michael J. ; Lopes, Luciene ; Ikeda, Yasuhiro H ; Salio, Mariolina ; Cerundolo, Vincenzo ; Collins, Mary K. / Intravenous Injection of a Lentiviral Vector Encoding NY-ESO-1 Induces an Effective CTL Response. In: Journal of Immunology. 2004 ; Vol. 172, No. 3. pp. 1582-1587.
@article{a59407cf293e4854ba0b2e2afed938eb,
title = "Intravenous Injection of a Lentiviral Vector Encoding NY-ESO-1 Induces an Effective CTL Response",
abstract = "Lentiviral vectors can efficiently transduce a variety of nondividing cells, including APCs. We assessed the immunogenicity of a lentiviral vector encoding the melanoma Ag NY-ESO-1 in HLA-A2 transgenic mice. Direct i.v. injection of NY-ESO-1 lentivirus induced NY-ESO-1157-165-specific CD8+ cells, detected ex vivo with an A2/H-2Kb chimeric class I tetramer. These NY-ESO-1157-165-specific CD8+ cells could be expanded by boosting with an NY-ESO-1 vaccinia virus and could kill NY-ESO-1157-165 peptide-pulsed targets in vivo. Such direct lentiviral vector injection was similar in potency to the injection of in vitro-transduced dendritic cells (DC). In addition, human monocyte-derived DC transduced by the NY-ESO-1 lentivirus stimulated an NY-ESO-1 157-165-specific specific CTL clone. These data suggest that direct lentiviral transduction of DC in vivo might provide a powerful immunotherapeutic strategy.",
author = "Palmowski, {Michael J.} and Luciene Lopes and Ikeda, {Yasuhiro H} and Mariolina Salio and Vincenzo Cerundolo and Collins, {Mary K.}",
year = "2004",
month = "2",
day = "1",
language = "English (US)",
volume = "172",
pages = "1582--1587",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "3",

}

TY - JOUR

T1 - Intravenous Injection of a Lentiviral Vector Encoding NY-ESO-1 Induces an Effective CTL Response

AU - Palmowski, Michael J.

AU - Lopes, Luciene

AU - Ikeda, Yasuhiro H

AU - Salio, Mariolina

AU - Cerundolo, Vincenzo

AU - Collins, Mary K.

PY - 2004/2/1

Y1 - 2004/2/1

N2 - Lentiviral vectors can efficiently transduce a variety of nondividing cells, including APCs. We assessed the immunogenicity of a lentiviral vector encoding the melanoma Ag NY-ESO-1 in HLA-A2 transgenic mice. Direct i.v. injection of NY-ESO-1 lentivirus induced NY-ESO-1157-165-specific CD8+ cells, detected ex vivo with an A2/H-2Kb chimeric class I tetramer. These NY-ESO-1157-165-specific CD8+ cells could be expanded by boosting with an NY-ESO-1 vaccinia virus and could kill NY-ESO-1157-165 peptide-pulsed targets in vivo. Such direct lentiviral vector injection was similar in potency to the injection of in vitro-transduced dendritic cells (DC). In addition, human monocyte-derived DC transduced by the NY-ESO-1 lentivirus stimulated an NY-ESO-1 157-165-specific specific CTL clone. These data suggest that direct lentiviral transduction of DC in vivo might provide a powerful immunotherapeutic strategy.

AB - Lentiviral vectors can efficiently transduce a variety of nondividing cells, including APCs. We assessed the immunogenicity of a lentiviral vector encoding the melanoma Ag NY-ESO-1 in HLA-A2 transgenic mice. Direct i.v. injection of NY-ESO-1 lentivirus induced NY-ESO-1157-165-specific CD8+ cells, detected ex vivo with an A2/H-2Kb chimeric class I tetramer. These NY-ESO-1157-165-specific CD8+ cells could be expanded by boosting with an NY-ESO-1 vaccinia virus and could kill NY-ESO-1157-165 peptide-pulsed targets in vivo. Such direct lentiviral vector injection was similar in potency to the injection of in vitro-transduced dendritic cells (DC). In addition, human monocyte-derived DC transduced by the NY-ESO-1 lentivirus stimulated an NY-ESO-1 157-165-specific specific CTL clone. These data suggest that direct lentiviral transduction of DC in vivo might provide a powerful immunotherapeutic strategy.

UR - http://www.scopus.com/inward/record.url?scp=1642444129&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1642444129&partnerID=8YFLogxK

M3 - Article

C2 - 14734738

AN - SCOPUS:1642444129

VL - 172

SP - 1582

EP - 1587

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 3

ER -