Intravascular delivery of biologics to the rat kidney

Federico Franchi, Xiang Yang Zhu, Tyra A. Witt, Lilach O Lerman, Martin G Rodriguez-Porcel

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The renal microvascular compartment plays an important role in the progression of kidney disease and hypertension, leading to the development of End Stage Renal Disease with high risk of death for cardiovascular events. Moreover, recent clinical studies have shown that renovascular structure and function may have a great impact on functional renal recovery after surgery. Here, we describe a protocol for the delivery of drugs into the renal artery of rats. This procedure offers significant advantages over the frequently used systemic administration as it may allow a more localized therapeutic effect. In addition, the use of rodents in pharmacodynamic analysis of preclinical studies may be cost effective, paving the way for the design of translational experiments in larger animal models. Using this technique, infusion of rat recombinant Vascular Endothelial Growth Factor (VEGF) protein in rats has induced activation of VEGF signaling as shown by increased expression of FLK1, pAKT/AKT, pERK/ ERK. In summary, we established a protocol for the intrarenal delivery of drugs in rats, which is simple and highly reproducible.

Original languageEnglish (US)
Article numbere54418
JournalJournal of Visualized Experiments
Volume2016
Issue number115
DOIs
StatePublished - Sep 1 2016

Fingerprint

Biological Products
Rats
Kidney
Vascular Endothelial Growth Factor A
Pharmacodynamics
Kidney Diseases
Therapeutic Uses
Renal Artery
Pharmaceutical Preparations
Design of experiments
Surgery
Chronic Kidney Failure
Rodentia
Animals
Animal Models
Chemical activation
Hypertension
Proteins
Costs and Cost Analysis
Recovery

Keywords

  • Cannulation
  • Issue 115
  • Kidney
  • Medicine
  • Rat intrarenal delivery
  • Renal artery
  • Renal disease
  • Renal microvasculature

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Intravascular delivery of biologics to the rat kidney. / Franchi, Federico; Zhu, Xiang Yang; Witt, Tyra A.; Lerman, Lilach O; Rodriguez-Porcel, Martin G.

In: Journal of Visualized Experiments, Vol. 2016, No. 115, e54418, 01.09.2016.

Research output: Contribution to journalArticle

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