Intratracheal administration of endotoxin and cytokines: VIII. LPS induces E-selectin expression; anti-E-selectin and soluble E-selectin inhibit acute inflammation

T. R. Ulich, S. C. Howard, D. G. Remick, E. S. Yi, T. Collins, K. Guo, S. Yin, J. L. Keene, J. J. Schmuke, C. N. Steininger, J. K. Welply, J. H. Williams

Research output: Contribution to journalArticle

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Abstract

E-selectin is an inducible endothelial adhesion molecule that binds neutrophils. E-selectin mRNA is not constitutively detectable in the lungs of rats. Intratracheal injection of LPS induces pulmonary E-selectin mRNA expression at 2-4 h. Intratracheal injection of LPS followed at 2 and 4 h by intravenous injection of mouse F(ab') 2 or F(ab') anti-E-selectin monoclonal antibody inhibits the emigration of neutrophils into the bronchoalveolar space at 6 h by 50-70% . TNF and IL-6 bioactivity are not decreased in bronchoalveolar lavage fluid after treatment with anti-E-selectin antibody as compare to controls suggesting that the anti-E-selectin does not affect the magnitude of the LPS-initiated cytokine cascade. Intratracheal injection of LPS followed at 2 and 4 h by intravenous injection of soluble E-selectin inhibits neutrophilic emigration at 6 h by 64%, suggesting that endogenous soluble E-selectin shed from activated endothelium may play a role in the endogenous down-regulation of acute inflammation. E-selectin-mediated adhesion of neutrophils to endothelium appears crucial to the full development of the acute inflammation response.

Original languageEnglish (US)
Pages (from-to)389-398
Number of pages10
JournalInflammation
Volume18
Issue number4
StatePublished - 1994
Externally publishedYes

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E-Selectin
Endotoxins
Cytokines
Inflammation
Neutrophils
Emigration and Immigration
Intravenous Injections
Injections
Endothelium
Lung
Messenger RNA
Bronchoalveolar Lavage Fluid
Interleukin-6
Down-Regulation
Monoclonal Antibodies
Antibodies

ASJC Scopus subject areas

  • Cell Biology
  • Immunology
  • Medicine(all)

Cite this

Ulich, T. R., Howard, S. C., Remick, D. G., Yi, E. S., Collins, T., Guo, K., ... Williams, J. H. (1994). Intratracheal administration of endotoxin and cytokines: VIII. LPS induces E-selectin expression; anti-E-selectin and soluble E-selectin inhibit acute inflammation. Inflammation, 18(4), 389-398.

Intratracheal administration of endotoxin and cytokines : VIII. LPS induces E-selectin expression; anti-E-selectin and soluble E-selectin inhibit acute inflammation. / Ulich, T. R.; Howard, S. C.; Remick, D. G.; Yi, E. S.; Collins, T.; Guo, K.; Yin, S.; Keene, J. L.; Schmuke, J. J.; Steininger, C. N.; Welply, J. K.; Williams, J. H.

In: Inflammation, Vol. 18, No. 4, 1994, p. 389-398.

Research output: Contribution to journalArticle

Ulich, TR, Howard, SC, Remick, DG, Yi, ES, Collins, T, Guo, K, Yin, S, Keene, JL, Schmuke, JJ, Steininger, CN, Welply, JK & Williams, JH 1994, 'Intratracheal administration of endotoxin and cytokines: VIII. LPS induces E-selectin expression; anti-E-selectin and soluble E-selectin inhibit acute inflammation', Inflammation, vol. 18, no. 4, pp. 389-398.
Ulich, T. R. ; Howard, S. C. ; Remick, D. G. ; Yi, E. S. ; Collins, T. ; Guo, K. ; Yin, S. ; Keene, J. L. ; Schmuke, J. J. ; Steininger, C. N. ; Welply, J. K. ; Williams, J. H. / Intratracheal administration of endotoxin and cytokines : VIII. LPS induces E-selectin expression; anti-E-selectin and soluble E-selectin inhibit acute inflammation. In: Inflammation. 1994 ; Vol. 18, No. 4. pp. 389-398.
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