Intrathymic administration of B cells induces prolonged survival of fully allogeneic cardiac grafts without prolonged deletion of donor-specific thymocytes

Masanori Niimi, Nick D. Jones, Octavio B. Pajaro, Peter J. Morris, Kathryn J. Wood

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Intrathymic (IT) injection of alloantigen has been shown to induce unresponsiveness to allografts although the exact mechanisms of tolerance induction remains unclear. C57BL/10 (H2b) cardiac allografts were accepted in C3H/He (H2(k)) mice pretreated with IT inoculation of donor splenocytes (1 x 106) in combination with a depleting anti-CD4 monoclonal antibody 27 days before cardiac transplantation. To investigate which cell types were responsible for tolerance induction by IT injection of alloantigen, resting B (rB) cells or dendritic cells were used as the thymic inoculum instead of whole splenocytes. IT injection of rB cells induced indefinite graft prolongation in all recipients while only 20% of mice that had received IT injection of dendritic cells accepted grafts for over 100 days. In contrast, IT injection of dendritic cells resulted in significant deletion of donor-specific thymocytes whereas rB cells were relatively ineffective. IT deletion is not essential for the induction of tolerance by IT injection of rB cells; nondeletional mechanisms can be involved.

Original languageEnglish (US)
Pages (from-to)177-181
Number of pages5
JournalTransplant Immunology
Volume6
Issue number3
DOIs
StatePublished - Sep 1998
Externally publishedYes

Fingerprint

Thymocytes
B-Lymphocytes
Transplants
Injections
Dendritic Cells
Isoantigens
Allografts
Heart Transplantation
Monoclonal Antibodies

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Transplantation

Cite this

Intrathymic administration of B cells induces prolonged survival of fully allogeneic cardiac grafts without prolonged deletion of donor-specific thymocytes. / Niimi, Masanori; Jones, Nick D.; Pajaro, Octavio B.; Morris, Peter J.; Wood, Kathryn J.

In: Transplant Immunology, Vol. 6, No. 3, 09.1998, p. 177-181.

Research output: Contribution to journalArticle

Niimi, Masanori ; Jones, Nick D. ; Pajaro, Octavio B. ; Morris, Peter J. ; Wood, Kathryn J. / Intrathymic administration of B cells induces prolonged survival of fully allogeneic cardiac grafts without prolonged deletion of donor-specific thymocytes. In: Transplant Immunology. 1998 ; Vol. 6, No. 3. pp. 177-181.
@article{9d355c8f2102468c9a81c826cee2ea18,
title = "Intrathymic administration of B cells induces prolonged survival of fully allogeneic cardiac grafts without prolonged deletion of donor-specific thymocytes",
abstract = "Intrathymic (IT) injection of alloantigen has been shown to induce unresponsiveness to allografts although the exact mechanisms of tolerance induction remains unclear. C57BL/10 (H2b) cardiac allografts were accepted in C3H/He (H2(k)) mice pretreated with IT inoculation of donor splenocytes (1 x 106) in combination with a depleting anti-CD4 monoclonal antibody 27 days before cardiac transplantation. To investigate which cell types were responsible for tolerance induction by IT injection of alloantigen, resting B (rB) cells or dendritic cells were used as the thymic inoculum instead of whole splenocytes. IT injection of rB cells induced indefinite graft prolongation in all recipients while only 20{\%} of mice that had received IT injection of dendritic cells accepted grafts for over 100 days. In contrast, IT injection of dendritic cells resulted in significant deletion of donor-specific thymocytes whereas rB cells were relatively ineffective. IT deletion is not essential for the induction of tolerance by IT injection of rB cells; nondeletional mechanisms can be involved.",
author = "Masanori Niimi and Jones, {Nick D.} and Pajaro, {Octavio B.} and Morris, {Peter J.} and Wood, {Kathryn J.}",
year = "1998",
month = "9",
doi = "10.1016/S0966-3274(98)80043-9",
language = "English (US)",
volume = "6",
pages = "177--181",
journal = "Transplant Immunology",
issn = "0966-3274",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Intrathymic administration of B cells induces prolonged survival of fully allogeneic cardiac grafts without prolonged deletion of donor-specific thymocytes

AU - Niimi, Masanori

AU - Jones, Nick D.

AU - Pajaro, Octavio B.

AU - Morris, Peter J.

AU - Wood, Kathryn J.

PY - 1998/9

Y1 - 1998/9

N2 - Intrathymic (IT) injection of alloantigen has been shown to induce unresponsiveness to allografts although the exact mechanisms of tolerance induction remains unclear. C57BL/10 (H2b) cardiac allografts were accepted in C3H/He (H2(k)) mice pretreated with IT inoculation of donor splenocytes (1 x 106) in combination with a depleting anti-CD4 monoclonal antibody 27 days before cardiac transplantation. To investigate which cell types were responsible for tolerance induction by IT injection of alloantigen, resting B (rB) cells or dendritic cells were used as the thymic inoculum instead of whole splenocytes. IT injection of rB cells induced indefinite graft prolongation in all recipients while only 20% of mice that had received IT injection of dendritic cells accepted grafts for over 100 days. In contrast, IT injection of dendritic cells resulted in significant deletion of donor-specific thymocytes whereas rB cells were relatively ineffective. IT deletion is not essential for the induction of tolerance by IT injection of rB cells; nondeletional mechanisms can be involved.

AB - Intrathymic (IT) injection of alloantigen has been shown to induce unresponsiveness to allografts although the exact mechanisms of tolerance induction remains unclear. C57BL/10 (H2b) cardiac allografts were accepted in C3H/He (H2(k)) mice pretreated with IT inoculation of donor splenocytes (1 x 106) in combination with a depleting anti-CD4 monoclonal antibody 27 days before cardiac transplantation. To investigate which cell types were responsible for tolerance induction by IT injection of alloantigen, resting B (rB) cells or dendritic cells were used as the thymic inoculum instead of whole splenocytes. IT injection of rB cells induced indefinite graft prolongation in all recipients while only 20% of mice that had received IT injection of dendritic cells accepted grafts for over 100 days. In contrast, IT injection of dendritic cells resulted in significant deletion of donor-specific thymocytes whereas rB cells were relatively ineffective. IT deletion is not essential for the induction of tolerance by IT injection of rB cells; nondeletional mechanisms can be involved.

UR - http://www.scopus.com/inward/record.url?scp=0031743822&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031743822&partnerID=8YFLogxK

U2 - 10.1016/S0966-3274(98)80043-9

DO - 10.1016/S0966-3274(98)80043-9

M3 - Article

C2 - 9848224

AN - SCOPUS:0031743822

VL - 6

SP - 177

EP - 181

JO - Transplant Immunology

JF - Transplant Immunology

SN - 0966-3274

IS - 3

ER -