Intrapulmonary cytokine accumulation following BAL and the role of endotoxin contamination

Michael E. Nelson, Tom C. Wald, Kirstin Bailey, Lewis J. Wesselius

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Study objectives: BAL induces alveolar inflammation, but its effects on intrapulmonary cytokines and the mechanisms causing inflammation are uncertain. The objectives of this study were: (1) to characterize cytokine response in the lungs to BAL, and (2) to determine whether endotoxin is introduced into the lungs during BAL, which could promote BAL-induced inflammation. Design and methods: We performed two BAL procedures in healthy volunteers separated by 4 (n = 6), 24 (n = 5), or 72 h (n = 3). The initial BAL was performed in the right middle lobe (RML) and the second BAL was performed in the same location and the lingula. Concentrations of interleukin-8 (IL-8), interleukin-1β (IL-1β), and transforming growth factor-β were measured by enzyme-linked immunosorbent assay and tumor necrosis factor-α (TNF-α) bioactivity was determined. Endotoxin contents of saline (10 and 20 mL) infused through bronchoscopes as well as BAL fluids recovered from six subjects were assessed by limulus amebocyte assay. Results: At 4 h after the initial lavage, but not at later times, BAL fluid recovered from the RML contained increased concentrations of IL-8 and IL- 1β, and increased TNF-α bioactivity. BAL fluid recovered from the lingula contained increased concentrations of TNF-α only at 4 h. All BAL samples tested contained detectable endotoxin as did all saline aliquots instilled through bronchoscopes. Conclusions: There is intrapulmonary accumulation of the cytokines TNF-α, IL-8, and IL-1β in the lavaged lung within 4 h after BAL; this accumulation resolves by 24 h. Endotoxin contamination of the lungs during bronchoscopy may contribute to BAL-induced lung inflammation.

Original languageEnglish (US)
Pages (from-to)151-157
Number of pages7
JournalChest
Volume115
Issue number1
DOIs
StatePublished - 1999
Externally publishedYes

Fingerprint

Dimercaprol
Endotoxins
Cytokines
Tumor Necrosis Factor-alpha
Interleukin-8
Interleukin-1
Bronchoscopes
Lung
Inflammation
Horseshoe Crabs
Therapeutic Irrigation
Transforming Growth Factors
Bronchoscopy

Keywords

  • Bronchoalveolar lavage
  • Cytokines
  • Endotoxin
  • Inflammation

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Intrapulmonary cytokine accumulation following BAL and the role of endotoxin contamination. / Nelson, Michael E.; Wald, Tom C.; Bailey, Kirstin; Wesselius, Lewis J.

In: Chest, Vol. 115, No. 1, 1999, p. 151-157.

Research output: Contribution to journalArticle

Nelson, ME, Wald, TC, Bailey, K & Wesselius, LJ 1999, 'Intrapulmonary cytokine accumulation following BAL and the role of endotoxin contamination', Chest, vol. 115, no. 1, pp. 151-157. https://doi.org/10.1378/chest.115.1.151
Nelson, Michael E. ; Wald, Tom C. ; Bailey, Kirstin ; Wesselius, Lewis J. / Intrapulmonary cytokine accumulation following BAL and the role of endotoxin contamination. In: Chest. 1999 ; Vol. 115, No. 1. pp. 151-157.
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