Intramuscular fatty acid metabolism evaluated with stable isotopic tracers

Zengkui Guo, Michael D. Jensen

Research output: Contribution to journalArticlepeer-review

49 Scopus citations


We evaluated the applicability of stable isotopic tracers to the study of intramuscular fatty acid metabolism by infusing both [U-13C]palmitate and [1-13C]oleate intravenously for 4 h into fasted conscious rats. Skeletal muscles were sequentially biopsied, and the concentration and 13C enrichment of fatty acids were measured by gas chromatography/combustion/isotope ratio mass spectrometry. Throughout the study, the 13C enrichment of plasma palmitate and oleate remained substantially greater than intramuscular nonesterified palmitate and oleate enrichment, which in turn was greater than intramuscular triglyceride palmitate and oleate enrichment. Fractional synthesis rates of intramuscular triglycerides in gastrocnemius and soleus were 0.267 ± 0.075 and 0.100 ± 0.030/h (P = 0.04), respectively, as determined by using [U-13C]palmitate, and were 0.278 ± 0.049 and 0.075 ± 0.013/h (P = 0.02), respectively, by using [1-13C]oleate. We conclude that plasma free fatty acids are a source for intramuscular triglycerides and nonesterified fatty acids; the latter are likely the synthetic precursors of the former. Uniformly and singly labeled [13C]fatty acid tracers will provide an important tool to study intramuscular fatty acid and triglyceride metabolism.

Original languageEnglish (US)
Pages (from-to)1674-1679
Number of pages6
JournalJournal of applied physiology
Issue number5
StatePublished - May 1998


  • Free fatty acids
  • Gas chromatography/combustion/isotope ratio mass spectrometry
  • Intramuscular triglycerides

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)


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