Intralesional Treatment of Established Murine Primary Renal Tumor With Interleukin-4: Localized Effect on Primary Tumor With no Impact on Metastases

Elia Younes, Gabriel P. Haas, Daniel W Visscher, J. Edson Pontes, Raj K. Puri, Gilda G. Hillman

Research output: Contribution to journalArticle

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Abstract

In an effort to develop new strategies for immunotherapy of metastatic renal cell carcinoma, we investigated the therapeutic potential of interleukin-4 in a visceral renal tumor using the murine Renca renal adenocarcinoma model. Renca cells were implanted underneath the renal capsule of Balb/c mice to induce a primary tumor that spontaneously metastasized to several organs. Established primary renal tumors 4 to 6 mm. in diameter were treated by intralesional administration of recombinant murine interleukin-4 (IL-4). This treatment caused a marked inhibition of the primary tumor growth but had little effect on the progression of metastases in the liver, mesentery and lungs. Immunohistochemistry studies performed on renal tumor sections showed a macrophage infiltration that became predominant 7 days after IL-4 treatment. CD8+ T cells were also observed at the periphery and within the tumor. These data suggest that IL-4 mediated a potent antitumor effect when administered intralesionally although its effects remained localized with no impact on metastases at distant sites. Interleukin-4 antitumor activity seems to be mediated by recruitment of macrophages and T cells in the tumor.

Original languageEnglish (US)
Pages (from-to)490-493
Number of pages4
JournalThe Journal of Urology
Volume153
Issue number2
DOIs
StatePublished - 1995
Externally publishedYes

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Interleukin-4
Neoplasm Metastasis
Kidney
Neoplasms
Renal Cell Carcinoma
Therapeutics
Macrophages
T-Lymphocytes
Mesentery
Immunotherapy
Capsules
Immunohistochemistry
Lung
Liver
Growth

ASJC Scopus subject areas

  • Urology

Cite this

Intralesional Treatment of Established Murine Primary Renal Tumor With Interleukin-4 : Localized Effect on Primary Tumor With no Impact on Metastases. / Younes, Elia; Haas, Gabriel P.; Visscher, Daniel W; Pontes, J. Edson; Puri, Raj K.; Hillman, Gilda G.

In: The Journal of Urology, Vol. 153, No. 2, 1995, p. 490-493.

Research output: Contribution to journalArticle

Younes, Elia ; Haas, Gabriel P. ; Visscher, Daniel W ; Pontes, J. Edson ; Puri, Raj K. ; Hillman, Gilda G. / Intralesional Treatment of Established Murine Primary Renal Tumor With Interleukin-4 : Localized Effect on Primary Tumor With no Impact on Metastases. In: The Journal of Urology. 1995 ; Vol. 153, No. 2. pp. 490-493.
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