Abstract
The physiological relevance of the absorption of glucose from bile by cholangiocytes remains unclear. The aim of this study was to test the hypothesis that absorbed glucose drives aquaporin (AQP)-mediated water transport by biliary epithelia and is thus involved in ductal bile formation. Glucose absorption and water transport by biliary epithelia were studied in vitro by microperfusing intrahepatic bile duct units (IBDUs) isolated from rat liver. In a separate set of in vivo experiments, bile flow and absorption of biliary glucose were measured after intraportal infusion of D-glucose or phlorizin. IBDUs absorbed D-glucose in a dose- and phlorizin-dependent manner with an absorption maximum of 92.8 ± 6.2 pmol·min-1·mm-1. Absorption of D-glucose by microperfused IBDUs resulted in an increase of water absorption (Jv = 3-10 nl·min-1·mm-1, Pf = 40 × 10-3 cm/sec). Glucose-driven water absorption by IBDUs was inhibited by HgCl2, suggesting that water passively follows absorbed D-glucose mainly transcellularly via mercury-sensitive AQPs. In vivo studies showed that as the amount of absorbed biliary glucose increased after intraportal infusion of D-glucose, bile flow decreased. In contrast, as the absorption of biliary glucose decreased after phlorizin, bile flow increased. Results support the hypothesis that the physiological significance of the absorption of biliary glucose by cholangiocytes is likely related to regulation of ductal bile formation.
| Original language | English (US) |
|---|---|
| Journal | American Journal of Physiology - Cell Physiology |
| Volume | 283 |
| Issue number | 3 52-3 |
| State | Published - Sep 2002 |
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Keywords
- Absorption
- Aquaporins
- Cholangiocytes
- Intrahepatic bile duct units
- Liver
- Microperfusion
- Phlorizin
- Secretion
ASJC Scopus subject areas
- Cell Biology
- Clinical Biochemistry
- Physiology
Cite this
Intrahepatic bile ducts transport water in response to absorbed glucose. / Masyuk, Anatoly I.; Masyuk, Tatyana V.; Tietz, Pamela S.; Splinter, Patrick L.; La Russo, Nicholas F.
In: American Journal of Physiology - Cell Physiology, Vol. 283, No. 3 52-3, 09.2002.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Intrahepatic bile ducts transport water in response to absorbed glucose
AU - Masyuk, Anatoly I.
AU - Masyuk, Tatyana V.
AU - Tietz, Pamela S.
AU - Splinter, Patrick L.
AU - La Russo, Nicholas F
PY - 2002/9
Y1 - 2002/9
N2 - The physiological relevance of the absorption of glucose from bile by cholangiocytes remains unclear. The aim of this study was to test the hypothesis that absorbed glucose drives aquaporin (AQP)-mediated water transport by biliary epithelia and is thus involved in ductal bile formation. Glucose absorption and water transport by biliary epithelia were studied in vitro by microperfusing intrahepatic bile duct units (IBDUs) isolated from rat liver. In a separate set of in vivo experiments, bile flow and absorption of biliary glucose were measured after intraportal infusion of D-glucose or phlorizin. IBDUs absorbed D-glucose in a dose- and phlorizin-dependent manner with an absorption maximum of 92.8 ± 6.2 pmol·min-1·mm-1. Absorption of D-glucose by microperfused IBDUs resulted in an increase of water absorption (Jv = 3-10 nl·min-1·mm-1, Pf = 40 × 10-3 cm/sec). Glucose-driven water absorption by IBDUs was inhibited by HgCl2, suggesting that water passively follows absorbed D-glucose mainly transcellularly via mercury-sensitive AQPs. In vivo studies showed that as the amount of absorbed biliary glucose increased after intraportal infusion of D-glucose, bile flow decreased. In contrast, as the absorption of biliary glucose decreased after phlorizin, bile flow increased. Results support the hypothesis that the physiological significance of the absorption of biliary glucose by cholangiocytes is likely related to regulation of ductal bile formation.
AB - The physiological relevance of the absorption of glucose from bile by cholangiocytes remains unclear. The aim of this study was to test the hypothesis that absorbed glucose drives aquaporin (AQP)-mediated water transport by biliary epithelia and is thus involved in ductal bile formation. Glucose absorption and water transport by biliary epithelia were studied in vitro by microperfusing intrahepatic bile duct units (IBDUs) isolated from rat liver. In a separate set of in vivo experiments, bile flow and absorption of biliary glucose were measured after intraportal infusion of D-glucose or phlorizin. IBDUs absorbed D-glucose in a dose- and phlorizin-dependent manner with an absorption maximum of 92.8 ± 6.2 pmol·min-1·mm-1. Absorption of D-glucose by microperfused IBDUs resulted in an increase of water absorption (Jv = 3-10 nl·min-1·mm-1, Pf = 40 × 10-3 cm/sec). Glucose-driven water absorption by IBDUs was inhibited by HgCl2, suggesting that water passively follows absorbed D-glucose mainly transcellularly via mercury-sensitive AQPs. In vivo studies showed that as the amount of absorbed biliary glucose increased after intraportal infusion of D-glucose, bile flow decreased. In contrast, as the absorption of biliary glucose decreased after phlorizin, bile flow increased. Results support the hypothesis that the physiological significance of the absorption of biliary glucose by cholangiocytes is likely related to regulation of ductal bile formation.
KW - Absorption
KW - Aquaporins
KW - Cholangiocytes
KW - Intrahepatic bile duct units
KW - Liver
KW - Microperfusion
KW - Phlorizin
KW - Secretion
UR - http://www.scopus.com/inward/record.url?scp=0036720935&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036720935&partnerID=8YFLogxK
M3 - Article
C2 - 12176735
AN - SCOPUS:0036720935
VL - 283
JO - American Journal of Physiology - Renal Fluid and Electrolyte Physiology
JF - American Journal of Physiology - Renal Fluid and Electrolyte Physiology
SN - 1931-857X
IS - 3 52-3
ER -