Intrahepatic bile ducts transport water in response to absorbed glucose

Anatoly I. Masyuk, Tatyana V. Masyuk, Pamela S. Tietz, Patrick L. Splinter, Nicholas F La Russo

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The physiological relevance of the absorption of glucose from bile by cholangiocytes remains unclear. The aim of this study was to test the hypothesis that absorbed glucose drives aquaporin (AQP)-mediated water transport by biliary epithelia and is thus involved in ductal bile formation. Glucose absorption and water transport by biliary epithelia were studied in vitro by microperfusing intrahepatic bile duct units (IBDUs) isolated from rat liver. In a separate set of in vivo experiments, bile flow and absorption of biliary glucose were measured after intraportal infusion of D-glucose or phlorizin. IBDUs absorbed D-glucose in a dose- and phlorizin-dependent manner with an absorption maximum of 92.8 ± 6.2 pmol·min-1·mm-1. Absorption of D-glucose by microperfused IBDUs resulted in an increase of water absorption (Jv = 3-10 nl·min-1·mm-1, Pf = 40 × 10-3 cm/sec). Glucose-driven water absorption by IBDUs was inhibited by HgCl2, suggesting that water passively follows absorbed D-glucose mainly transcellularly via mercury-sensitive AQPs. In vivo studies showed that as the amount of absorbed biliary glucose increased after intraportal infusion of D-glucose, bile flow decreased. In contrast, as the absorption of biliary glucose decreased after phlorizin, bile flow increased. Results support the hypothesis that the physiological significance of the absorption of biliary glucose by cholangiocytes is likely related to regulation of ductal bile formation.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume283
Issue number3 52-3
StatePublished - Sep 2002

Fingerprint

Intrahepatic Bile Ducts
Ducts
Glucose
Water
Bile
Phlorhizin
Water absorption
Epithelium
Mercuric Chloride
Aquaporins
Mercury
Liver
Rats

Keywords

  • Absorption
  • Aquaporins
  • Cholangiocytes
  • Intrahepatic bile duct units
  • Liver
  • Microperfusion
  • Phlorizin
  • Secretion

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

Cite this

Intrahepatic bile ducts transport water in response to absorbed glucose. / Masyuk, Anatoly I.; Masyuk, Tatyana V.; Tietz, Pamela S.; Splinter, Patrick L.; La Russo, Nicholas F.

In: American Journal of Physiology - Cell Physiology, Vol. 283, No. 3 52-3, 09.2002.

Research output: Contribution to journalArticle

Masyuk, Anatoly I. ; Masyuk, Tatyana V. ; Tietz, Pamela S. ; Splinter, Patrick L. ; La Russo, Nicholas F. / Intrahepatic bile ducts transport water in response to absorbed glucose. In: American Journal of Physiology - Cell Physiology. 2002 ; Vol. 283, No. 3 52-3.
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