Intragenic recombination in an E(β) gene for a murine Ia antigen

M. L. Plunkett, J. E. Coligan, C. S. David, J. H. Freed

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The recombinant strain D2.GD was originally typed as I-A(d) by serological methods. Indeed, the A(α) and A(β) chains of the I-A antigens appear to exhibit normal behavior by the criteria of serology and two dimensional gel analysis. However, the E(β) chain encoded by the I-A subregion of this strain, one of the two components of the plasma membrane located I-E antigens produced in (D2.GD x A.TFR5)F 1 animals, has been demonstrated to be the product of an intragenic recombinational event between E(β) genes from the d and b haplotypes. Sequence analysis suggests that the amino-terminal portion of the E(β)(g2) chain is derived from the d haplotype and, therefore, that the coding strand for this gene is oriented centromeric → telomeric (5' to 3' direction). Finally, these data combined with the data of Rose and Cullen allow the ordering of the genes within the I-A subregion as (H-2K), A(α), A(β), E(β) ... (H-2D).

Original languageEnglish (US)
Pages (from-to)937-942
Number of pages6
JournalJournal of Experimental Medicine
Volume155
Issue number3
StatePublished - 1982
Externally publishedYes

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Histocompatibility Antigens Class II
Genetic Recombination
Haplotypes
Genes
Serology
Sequence Analysis
Gels
Cell Membrane

ASJC Scopus subject areas

  • Immunology

Cite this

Plunkett, M. L., Coligan, J. E., David, C. S., & Freed, J. H. (1982). Intragenic recombination in an E(β) gene for a murine Ia antigen. Journal of Experimental Medicine, 155(3), 937-942.

Intragenic recombination in an E(β) gene for a murine Ia antigen. / Plunkett, M. L.; Coligan, J. E.; David, C. S.; Freed, J. H.

In: Journal of Experimental Medicine, Vol. 155, No. 3, 1982, p. 937-942.

Research output: Contribution to journalArticle

Plunkett, ML, Coligan, JE, David, CS & Freed, JH 1982, 'Intragenic recombination in an E(β) gene for a murine Ia antigen', Journal of Experimental Medicine, vol. 155, no. 3, pp. 937-942.
Plunkett ML, Coligan JE, David CS, Freed JH. Intragenic recombination in an E(β) gene for a murine Ia antigen. Journal of Experimental Medicine. 1982;155(3):937-942.
Plunkett, M. L. ; Coligan, J. E. ; David, C. S. ; Freed, J. H. / Intragenic recombination in an E(β) gene for a murine Ia antigen. In: Journal of Experimental Medicine. 1982 ; Vol. 155, No. 3. pp. 937-942.
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