Intracellular calcium transients in human working myocardium as detected with aequorin

J. P. Morgan, J. H. Chesebro, J. R. Pluth, F. J. Puga, Hartzell V Schaff

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The calcium transients associated with contraction in human working myocardium were recorded by use of the bioluminescent protein, aequorin, a substance that emits light when it combines with calcium ion (Ca++). Small amounts of aequorin were microinjected into superficial cells of human atrial and ventricular muscle obtained from tissue routinely excised and discarded at the time of cardiac surgery. Light output, an index of intracellular Ca++, and isometric tension development were recorded at 37.5°C at 1 to 5 second intervals of stimulation. Light increases much more quickly than tension and decreases toward basal levels by the time that peak tension is reached. The configuration and time course of the aequorin signal in human myocardium and its responses to inotropic interventions are similar to those recorded in lower mammalian species. The calcium transient appears to be dominated by the release and uptake of Ca++ from intracellular stores under all conditions studied. These results indicate that aequorin is a useful tool for studying the effects of drugs and disease states on cardiac excitation-contraction coupling in human beings as well as in lower animals.

Original languageEnglish (US)
Pages (from-to)410-418
Number of pages9
JournalJournal of the American College of Cardiology
Volume3
Issue number2 I
DOIs
StatePublished - 1984

Fingerprint

Aequorin
Myocardium
Calcium
Light
Luminescent Proteins
Excitation Contraction Coupling
Thoracic Surgery
Ions
Muscles
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Nursing(all)

Cite this

Intracellular calcium transients in human working myocardium as detected with aequorin. / Morgan, J. P.; Chesebro, J. H.; Pluth, J. R.; Puga, F. J.; Schaff, Hartzell V.

In: Journal of the American College of Cardiology, Vol. 3, No. 2 I, 1984, p. 410-418.

Research output: Contribution to journalArticle

Morgan, J. P. ; Chesebro, J. H. ; Pluth, J. R. ; Puga, F. J. ; Schaff, Hartzell V. / Intracellular calcium transients in human working myocardium as detected with aequorin. In: Journal of the American College of Cardiology. 1984 ; Vol. 3, No. 2 I. pp. 410-418.
@article{292e6f5e3da1430e98e17530b28351f0,
title = "Intracellular calcium transients in human working myocardium as detected with aequorin",
abstract = "The calcium transients associated with contraction in human working myocardium were recorded by use of the bioluminescent protein, aequorin, a substance that emits light when it combines with calcium ion (Ca++). Small amounts of aequorin were microinjected into superficial cells of human atrial and ventricular muscle obtained from tissue routinely excised and discarded at the time of cardiac surgery. Light output, an index of intracellular Ca++, and isometric tension development were recorded at 37.5°C at 1 to 5 second intervals of stimulation. Light increases much more quickly than tension and decreases toward basal levels by the time that peak tension is reached. The configuration and time course of the aequorin signal in human myocardium and its responses to inotropic interventions are similar to those recorded in lower mammalian species. The calcium transient appears to be dominated by the release and uptake of Ca++ from intracellular stores under all conditions studied. These results indicate that aequorin is a useful tool for studying the effects of drugs and disease states on cardiac excitation-contraction coupling in human beings as well as in lower animals.",
author = "Morgan, {J. P.} and Chesebro, {J. H.} and Pluth, {J. R.} and Puga, {F. J.} and Schaff, {Hartzell V}",
year = "1984",
doi = "10.1016/S0735-1097(84)80028-5",
language = "English (US)",
volume = "3",
pages = "410--418",
journal = "Journal of the American College of Cardiology",
issn = "0735-1097",
publisher = "Elsevier USA",
number = "2 I",

}

TY - JOUR

T1 - Intracellular calcium transients in human working myocardium as detected with aequorin

AU - Morgan, J. P.

AU - Chesebro, J. H.

AU - Pluth, J. R.

AU - Puga, F. J.

AU - Schaff, Hartzell V

PY - 1984

Y1 - 1984

N2 - The calcium transients associated with contraction in human working myocardium were recorded by use of the bioluminescent protein, aequorin, a substance that emits light when it combines with calcium ion (Ca++). Small amounts of aequorin were microinjected into superficial cells of human atrial and ventricular muscle obtained from tissue routinely excised and discarded at the time of cardiac surgery. Light output, an index of intracellular Ca++, and isometric tension development were recorded at 37.5°C at 1 to 5 second intervals of stimulation. Light increases much more quickly than tension and decreases toward basal levels by the time that peak tension is reached. The configuration and time course of the aequorin signal in human myocardium and its responses to inotropic interventions are similar to those recorded in lower mammalian species. The calcium transient appears to be dominated by the release and uptake of Ca++ from intracellular stores under all conditions studied. These results indicate that aequorin is a useful tool for studying the effects of drugs and disease states on cardiac excitation-contraction coupling in human beings as well as in lower animals.

AB - The calcium transients associated with contraction in human working myocardium were recorded by use of the bioluminescent protein, aequorin, a substance that emits light when it combines with calcium ion (Ca++). Small amounts of aequorin were microinjected into superficial cells of human atrial and ventricular muscle obtained from tissue routinely excised and discarded at the time of cardiac surgery. Light output, an index of intracellular Ca++, and isometric tension development were recorded at 37.5°C at 1 to 5 second intervals of stimulation. Light increases much more quickly than tension and decreases toward basal levels by the time that peak tension is reached. The configuration and time course of the aequorin signal in human myocardium and its responses to inotropic interventions are similar to those recorded in lower mammalian species. The calcium transient appears to be dominated by the release and uptake of Ca++ from intracellular stores under all conditions studied. These results indicate that aequorin is a useful tool for studying the effects of drugs and disease states on cardiac excitation-contraction coupling in human beings as well as in lower animals.

UR - http://www.scopus.com/inward/record.url?scp=0021340898&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021340898&partnerID=8YFLogxK

U2 - 10.1016/S0735-1097(84)80028-5

DO - 10.1016/S0735-1097(84)80028-5

M3 - Article

C2 - 6319470

AN - SCOPUS:0021340898

VL - 3

SP - 410

EP - 418

JO - Journal of the American College of Cardiology

JF - Journal of the American College of Cardiology

SN - 0735-1097

IS - 2 I

ER -