TY - JOUR
T1 - Intra-operatively obtained human tissue
T2 - Protocols and techniques for the study of neural stem cells
AU - Chaichana, Kaisorn L.
AU - Guerrero-Cazares, Hugo
AU - Capilla-Gonzalez, Vivian
AU - Zamora-Berridi, Grettel
AU - Achanta, Pragathi
AU - Gonzalez-Perez, Oscar
AU - Jallo, George I.
AU - Garcia-Verdugo, Jose Manuel
AU - Quiñones-Hinojosa, Alfredo
N1 - Funding Information:
We would like to thank Roxana Mesias, Ashwini Niranjan, and Alyssa Choi for their help in maintaining cell cultures and taking images. Kaisorn L. Chaichana was funded by the American Brain Tumor Association for some of the work conducted in this paper. This work was funded by NIH Grant K08NS055851 to A.Q.H., H.G.C., and G.I.J. were funded by the Children's Cancer Foundation. G.Z.B. was funded by the HHMI. O.G.P. was partially supported by FRABA Grant 554/08. J.M.G.V. was funded by the RETIC grant.
PY - 2009/5/30
Y1 - 2009/5/30
N2 - The discoveries of neural (NSCs) and brain tumor stem cells (BTSCs) in the adult human brain and in brain tumors, respectively, have led to a new era in neuroscience research. These cells represent novel approaches to studying normal phenomena such as memory and learning, as well as pathological conditions such as Parkinson's disease, stroke, and brain tumors. This new paradigm stresses the importance of understanding how these cells behave in vitro and in vivo. It also stresses the need to use human-derived tissue to study human disease because animal models may not necessarily accurately replicate the processes that occur in humans. An important, but often underused, source of human tissue and, consequently, both NSCs and BTSCs, is the operating room. This study describes in detail both current and newly developed laboratory techniques, which in our experience are used to process and study human NSCs and BTSCs from tissue obtained directly from the operating room.
AB - The discoveries of neural (NSCs) and brain tumor stem cells (BTSCs) in the adult human brain and in brain tumors, respectively, have led to a new era in neuroscience research. These cells represent novel approaches to studying normal phenomena such as memory and learning, as well as pathological conditions such as Parkinson's disease, stroke, and brain tumors. This new paradigm stresses the importance of understanding how these cells behave in vitro and in vivo. It also stresses the need to use human-derived tissue to study human disease because animal models may not necessarily accurately replicate the processes that occur in humans. An important, but often underused, source of human tissue and, consequently, both NSCs and BTSCs, is the operating room. This study describes in detail both current and newly developed laboratory techniques, which in our experience are used to process and study human NSCs and BTSCs from tissue obtained directly from the operating room.
KW - Brain tumor stem cells
KW - Electron microscopy
KW - Immunocytochemistry
KW - Immunohistochemistry
KW - Neural stem cells
KW - Neurospheres
UR - http://www.scopus.com/inward/record.url?scp=67349171375&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=67349171375&partnerID=8YFLogxK
U2 - 10.1016/j.jneumeth.2009.02.014
DO - 10.1016/j.jneumeth.2009.02.014
M3 - Article
C2 - 19427538
AN - SCOPUS:67349171375
SN - 0165-0270
VL - 180
SP - 116
EP - 125
JO - Journal of Neuroscience Methods
JF - Journal of Neuroscience Methods
IS - 1
ER -