Intra-articular implantation of collagen scaffold carriers is safe in both native and arthrofibrotic rabbit knee joints

J. A. Walker, T. J. Ewald, E. Lewallen, Andre J van Wijnen, A. D. Hanssen, B. F. Morrey, M. E. Morrey, Matthew Abdel, J. Sanchez-Sotelo

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Objectives: sustained intra-articular delivery of pharmacological agents is an attractive modality but requires use of a safe carrier that would not induce cartilage damage or fibrosis. collagen scaffolds are widely available and could be used intra-articularly, but no investigation has looked at the safety of collagen scaffolds within synovial joints. The aim of this study was to determine the safety of collagen scaffold implantation in a validated in vivo animal model of knee arthrofibrosis. Materials and Methods: A total of 96 rabbits were randomly and equally assigned to four different groups: arthrotomy alone; arthrotomy and collagen scaffold placement; contracture surgery; and contracture surgery and collagen scaffold placement. Animals were killed in equal numbers at 72 hours, two weeks, eight weeks, and 24 weeks. Joint contracture was measured, and cartilage and synovial samples underwent histological analysis. Results: Animals that underwent arthrotomy had equivalent joint contractures regardless of scaffold implantation (-13.9° versus-10.9°, equivalence limit 15°). Animals that underwent surgery to induce contracture did not demonstrate equivalent joint contractures with (41.8°) or without (53.9°) collagen scaffold implantation. chondral damage occurred in similar rates with (11 of 48) and without (nine of 48) scaffold implantation. no significant difference in synovitis was noted between groups. Absorption of the collagen scaffold occurred within eight weeks in all animals Conclusion: our data suggest that intra-articular implantation of a collagen sponge does not induce synovitis or cartilage damage. Implantation in a native joint does not seem to induce contracture. Implantation of the collagen sponge in a rabbit knee model of contracture may decrease the severity of the contracture.

Original languageEnglish (US)
Pages (from-to)162-171
Number of pages10
JournalBone and Joint Research
Volume6
Issue number3
DOIs
StatePublished - Mar 1 2017

Fingerprint

Contracture
Knee Joint
Collagen
Joints
Rabbits
Cartilage
Synovitis
Porifera
Knee
Safety
Fibrosis
Animal Models
Pharmacology

Keywords

  • Arthrofbrosis
  • Biocompatibility
  • Biodegradation
  • Collagen scafold
  • Knee joint

ASJC Scopus subject areas

  • Surgery
  • Orthopedics and Sports Medicine

Cite this

Walker, J. A., Ewald, T. J., Lewallen, E., van Wijnen, A. J., Hanssen, A. D., Morrey, B. F., ... Sanchez-Sotelo, J. (2017). Intra-articular implantation of collagen scaffold carriers is safe in both native and arthrofibrotic rabbit knee joints. Bone and Joint Research, 6(3), 162-171. https://doi.org/10.1302/2046-3758.63.BJR-2016.0193

Intra-articular implantation of collagen scaffold carriers is safe in both native and arthrofibrotic rabbit knee joints. / Walker, J. A.; Ewald, T. J.; Lewallen, E.; van Wijnen, Andre J; Hanssen, A. D.; Morrey, B. F.; Morrey, M. E.; Abdel, Matthew; Sanchez-Sotelo, J.

In: Bone and Joint Research, Vol. 6, No. 3, 01.03.2017, p. 162-171.

Research output: Contribution to journalArticle

Walker, JA, Ewald, TJ, Lewallen, E, van Wijnen, AJ, Hanssen, AD, Morrey, BF, Morrey, ME, Abdel, M & Sanchez-Sotelo, J 2017, 'Intra-articular implantation of collagen scaffold carriers is safe in both native and arthrofibrotic rabbit knee joints', Bone and Joint Research, vol. 6, no. 3, pp. 162-171. https://doi.org/10.1302/2046-3758.63.BJR-2016.0193
Walker, J. A. ; Ewald, T. J. ; Lewallen, E. ; van Wijnen, Andre J ; Hanssen, A. D. ; Morrey, B. F. ; Morrey, M. E. ; Abdel, Matthew ; Sanchez-Sotelo, J. / Intra-articular implantation of collagen scaffold carriers is safe in both native and arthrofibrotic rabbit knee joints. In: Bone and Joint Research. 2017 ; Vol. 6, No. 3. pp. 162-171.
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AU - Ewald, T. J.

AU - Lewallen, E.

AU - van Wijnen, Andre J

AU - Hanssen, A. D.

AU - Morrey, B. F.

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AU - Sanchez-Sotelo, J.

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N2 - Objectives: sustained intra-articular delivery of pharmacological agents is an attractive modality but requires use of a safe carrier that would not induce cartilage damage or fibrosis. collagen scaffolds are widely available and could be used intra-articularly, but no investigation has looked at the safety of collagen scaffolds within synovial joints. The aim of this study was to determine the safety of collagen scaffold implantation in a validated in vivo animal model of knee arthrofibrosis. Materials and Methods: A total of 96 rabbits were randomly and equally assigned to four different groups: arthrotomy alone; arthrotomy and collagen scaffold placement; contracture surgery; and contracture surgery and collagen scaffold placement. Animals were killed in equal numbers at 72 hours, two weeks, eight weeks, and 24 weeks. Joint contracture was measured, and cartilage and synovial samples underwent histological analysis. Results: Animals that underwent arthrotomy had equivalent joint contractures regardless of scaffold implantation (-13.9° versus-10.9°, equivalence limit 15°). Animals that underwent surgery to induce contracture did not demonstrate equivalent joint contractures with (41.8°) or without (53.9°) collagen scaffold implantation. chondral damage occurred in similar rates with (11 of 48) and without (nine of 48) scaffold implantation. no significant difference in synovitis was noted between groups. Absorption of the collagen scaffold occurred within eight weeks in all animals Conclusion: our data suggest that intra-articular implantation of a collagen sponge does not induce synovitis or cartilage damage. Implantation in a native joint does not seem to induce contracture. Implantation of the collagen sponge in a rabbit knee model of contracture may decrease the severity of the contracture.

AB - Objectives: sustained intra-articular delivery of pharmacological agents is an attractive modality but requires use of a safe carrier that would not induce cartilage damage or fibrosis. collagen scaffolds are widely available and could be used intra-articularly, but no investigation has looked at the safety of collagen scaffolds within synovial joints. The aim of this study was to determine the safety of collagen scaffold implantation in a validated in vivo animal model of knee arthrofibrosis. Materials and Methods: A total of 96 rabbits were randomly and equally assigned to four different groups: arthrotomy alone; arthrotomy and collagen scaffold placement; contracture surgery; and contracture surgery and collagen scaffold placement. Animals were killed in equal numbers at 72 hours, two weeks, eight weeks, and 24 weeks. Joint contracture was measured, and cartilage and synovial samples underwent histological analysis. Results: Animals that underwent arthrotomy had equivalent joint contractures regardless of scaffold implantation (-13.9° versus-10.9°, equivalence limit 15°). Animals that underwent surgery to induce contracture did not demonstrate equivalent joint contractures with (41.8°) or without (53.9°) collagen scaffold implantation. chondral damage occurred in similar rates with (11 of 48) and without (nine of 48) scaffold implantation. no significant difference in synovitis was noted between groups. Absorption of the collagen scaffold occurred within eight weeks in all animals Conclusion: our data suggest that intra-articular implantation of a collagen sponge does not induce synovitis or cartilage damage. Implantation in a native joint does not seem to induce contracture. Implantation of the collagen sponge in a rabbit knee model of contracture may decrease the severity of the contracture.

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KW - Biocompatibility

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