Intra-articular gene delivery and expression of interleukin-1Ra mediated by self-complementary adeno-associated virus

Jesse D. Kay, Elvire Gouze, Thomas J. Oligino, Jean Noel Gouze, Rachel S. Watson, Padraic P. Levings, Marsha L. Bush, Anthony Dacanay, David M. Nickerson, Paul D. Robbins, Christopher H Evans, Steven C. Ghivizzani

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Background: The adeno-associated virus (AAV) has many safety features that favor its use in the treatment of arthritic conditions; however, the conventional, single-stranded vector is inefficient for gene delivery to fibroblastic cells that primarily populate articular tissues. This has been attributed to the inability of these cells to convert the vector to a double-stranded form. To overcome this, we evaluated double-stranded self-complementary (sc) AAV as a vehicle for intra-articular gene delivery. Methods: Conventional and scAAV vectors were used to infect lapine articular fibroblasts in culture to determine transduction efficiency, transgene expression levels, and nuclear trafficking. scAAV containing the cDNA for interleukin (IL)-1 receptor antagonist (Ra) was delivered to the joints of naïve rabbits and those with IL-1β-induced arthritis. From lavage of the joint space, levels of transgenic expression and persistence were measured by enzyme-linked immunosorbent assay. Infiltrating leukocytes were quantified using a hemocytometer. Results: Transgene expression from scAAV had an earlier onset and was approximately 25-fold greater than conventional AAV despite the presence of similar numbers of viral genomes in the nuclei of infected cells. Fibroblasts transduced with scAAV produced amounts of IL1-Ra comparable to those transduced with adenoviral and lentiviral vectors. IL1-Ra was present in lavage fluid of most animals for 2 weeks in sufficient quantities to inhibit inflammation of the IL-1β-driven model. Once lost, neither subsequent inflammatory events, nor re-administration of the virus could re-establish transgene expression. Conclusions: scAAV-mediated intra-articular gene transfer is robust and similarly efficient in both normal and inflamed joints; the resulting transgenic expression is sufficient to achieve biological relevance in joints of human proportion.

Original languageEnglish (US)
Pages (from-to)605-614
Number of pages10
JournalJournal of Gene Medicine
Volume11
Issue number7
DOIs
StatePublished - 2009
Externally publishedYes

Fingerprint

Dependovirus
Interleukins
Joints
Gene Expression
Interleukin-1 Receptors
Transgenes
Therapeutic Irrigation
Interleukin-1
Arthritis
Fibroblasts
Genes
Viral Genome
Cell Nucleus
Leukocytes
Complementary DNA
Enzyme-Linked Immunosorbent Assay
Rabbits
Viruses
Inflammation
Safety

Keywords

  • Adeno-associated virus
  • Arthritis
  • Gene therapy
  • Interleukin-1
  • Interleukin-1 receptor antagonist

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Molecular Medicine
  • Genetics(clinical)
  • Drug Discovery
  • Medicine(all)

Cite this

Kay, J. D., Gouze, E., Oligino, T. J., Gouze, J. N., Watson, R. S., Levings, P. P., ... Ghivizzani, S. C. (2009). Intra-articular gene delivery and expression of interleukin-1Ra mediated by self-complementary adeno-associated virus. Journal of Gene Medicine, 11(7), 605-614. https://doi.org/10.1002/jgm.1334

Intra-articular gene delivery and expression of interleukin-1Ra mediated by self-complementary adeno-associated virus. / Kay, Jesse D.; Gouze, Elvire; Oligino, Thomas J.; Gouze, Jean Noel; Watson, Rachel S.; Levings, Padraic P.; Bush, Marsha L.; Dacanay, Anthony; Nickerson, David M.; Robbins, Paul D.; Evans, Christopher H; Ghivizzani, Steven C.

In: Journal of Gene Medicine, Vol. 11, No. 7, 2009, p. 605-614.

Research output: Contribution to journalArticle

Kay, JD, Gouze, E, Oligino, TJ, Gouze, JN, Watson, RS, Levings, PP, Bush, ML, Dacanay, A, Nickerson, DM, Robbins, PD, Evans, CH & Ghivizzani, SC 2009, 'Intra-articular gene delivery and expression of interleukin-1Ra mediated by self-complementary adeno-associated virus', Journal of Gene Medicine, vol. 11, no. 7, pp. 605-614. https://doi.org/10.1002/jgm.1334
Kay, Jesse D. ; Gouze, Elvire ; Oligino, Thomas J. ; Gouze, Jean Noel ; Watson, Rachel S. ; Levings, Padraic P. ; Bush, Marsha L. ; Dacanay, Anthony ; Nickerson, David M. ; Robbins, Paul D. ; Evans, Christopher H ; Ghivizzani, Steven C. / Intra-articular gene delivery and expression of interleukin-1Ra mediated by self-complementary adeno-associated virus. In: Journal of Gene Medicine. 2009 ; Vol. 11, No. 7. pp. 605-614.
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abstract = "Background: The adeno-associated virus (AAV) has many safety features that favor its use in the treatment of arthritic conditions; however, the conventional, single-stranded vector is inefficient for gene delivery to fibroblastic cells that primarily populate articular tissues. This has been attributed to the inability of these cells to convert the vector to a double-stranded form. To overcome this, we evaluated double-stranded self-complementary (sc) AAV as a vehicle for intra-articular gene delivery. Methods: Conventional and scAAV vectors were used to infect lapine articular fibroblasts in culture to determine transduction efficiency, transgene expression levels, and nuclear trafficking. scAAV containing the cDNA for interleukin (IL)-1 receptor antagonist (Ra) was delivered to the joints of na{\"i}ve rabbits and those with IL-1β-induced arthritis. From lavage of the joint space, levels of transgenic expression and persistence were measured by enzyme-linked immunosorbent assay. Infiltrating leukocytes were quantified using a hemocytometer. Results: Transgene expression from scAAV had an earlier onset and was approximately 25-fold greater than conventional AAV despite the presence of similar numbers of viral genomes in the nuclei of infected cells. Fibroblasts transduced with scAAV produced amounts of IL1-Ra comparable to those transduced with adenoviral and lentiviral vectors. IL1-Ra was present in lavage fluid of most animals for 2 weeks in sufficient quantities to inhibit inflammation of the IL-1β-driven model. Once lost, neither subsequent inflammatory events, nor re-administration of the virus could re-establish transgene expression. Conclusions: scAAV-mediated intra-articular gene transfer is robust and similarly efficient in both normal and inflamed joints; the resulting transgenic expression is sufficient to achieve biological relevance in joints of human proportion.",
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AU - Kay, Jesse D.

AU - Gouze, Elvire

AU - Oligino, Thomas J.

AU - Gouze, Jean Noel

AU - Watson, Rachel S.

AU - Levings, Padraic P.

AU - Bush, Marsha L.

AU - Dacanay, Anthony

AU - Nickerson, David M.

AU - Robbins, Paul D.

AU - Evans, Christopher H

AU - Ghivizzani, Steven C.

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KW - Arthritis

KW - Gene therapy

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KW - Interleukin-1 receptor antagonist

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