Intestinal microbiota metabolism of l-carnitine, a nutrient in red meat, promotes atherosclerosis

Robert A. Koeth, Zeneng Wang, Bruce S. Levison, Jennifer A. Buffa, Elin Org, Brendan T. Sheehy, Earl B. Britt, Xiaoming Fu, Yuping Wu, Lin Li, Jonathan D. Smith, Joseph A. Didonato, Jun Chen, Hongzhe Li, Gary D. Wu, James D. Lewis, Manya Warrier, J. Mark Brown, Ronald M. Krauss, W. H Wilson TangFrederic D. Bushman, Aldons J. Lusis, Stanley L. Hazen

Research output: Contribution to journalArticle

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Abstract

Intestinal microbiota metabolism of choline and phosphatidylcholine produces trimethylamine (TMA), which is further metabolized to a proatherogenic species, trimethylamine-N-oxide (TMAO). We demonstrate here that metabolism by intestinal microbiota of dietary l-carnitine, a trimethylamine abundant in red meat, also produces TMAO and accelerates atherosclerosis in mice. Omnivorous human subjects produced more TMAO than did vegans or vegetarians following ingestion of l-carnitine through a microbiota-dependent mechanism. The presence of specific bacterial taxa in human feces was associated with both plasma TMAO concentration and dietary status. Plasma l-carnitine levels in subjects undergoing cardiac evaluation (n = 2,595) predicted increased risks for both prevalent cardiovascular disease (CVD) and incident major adverse cardiac events (myocardial infarction, stroke or death), but only among subjects with concurrently high TMAO levels. Chronic dietary l-carnitine supplementation in mice altered cecal microbial composition, markedly enhanced synthesis of TMA and TMAO, and increased atherosclerosis, but this did not occur if intestinal microbiota was concurrently suppressed. In mice with an intact intestinal microbiota, dietary supplementation with TMAO or either carnitine or choline reduced in vivo reverse cholesterol transport. Intestinal microbiota may thus contribute to the well-established link between high levels of red meat consumption and CVD risk.

Original languageEnglish (US)
Pages (from-to)576-585
Number of pages10
JournalNature Medicine
Volume19
Issue number5
DOIs
StatePublished - May 2013
Externally publishedYes

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Carnitine
Meats
Metabolism
Nutrients
Atherosclerosis
Food
Choline
Cardiovascular Diseases
Plasmas
Microbiota
Dietary Supplements
trimethyloxamine
Gastrointestinal Microbiome
Red Meat
Phosphatidylcholines
Feces
Eating
Stroke
Myocardial Infarction
Cholesterol

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Koeth, R. A., Wang, Z., Levison, B. S., Buffa, J. A., Org, E., Sheehy, B. T., ... Hazen, S. L. (2013). Intestinal microbiota metabolism of l-carnitine, a nutrient in red meat, promotes atherosclerosis. Nature Medicine, 19(5), 576-585. https://doi.org/10.1038/nm.3145

Intestinal microbiota metabolism of l-carnitine, a nutrient in red meat, promotes atherosclerosis. / Koeth, Robert A.; Wang, Zeneng; Levison, Bruce S.; Buffa, Jennifer A.; Org, Elin; Sheehy, Brendan T.; Britt, Earl B.; Fu, Xiaoming; Wu, Yuping; Li, Lin; Smith, Jonathan D.; Didonato, Joseph A.; Chen, Jun; Li, Hongzhe; Wu, Gary D.; Lewis, James D.; Warrier, Manya; Brown, J. Mark; Krauss, Ronald M.; Tang, W. H Wilson; Bushman, Frederic D.; Lusis, Aldons J.; Hazen, Stanley L.

In: Nature Medicine, Vol. 19, No. 5, 05.2013, p. 576-585.

Research output: Contribution to journalArticle

Koeth, RA, Wang, Z, Levison, BS, Buffa, JA, Org, E, Sheehy, BT, Britt, EB, Fu, X, Wu, Y, Li, L, Smith, JD, Didonato, JA, Chen, J, Li, H, Wu, GD, Lewis, JD, Warrier, M, Brown, JM, Krauss, RM, Tang, WHW, Bushman, FD, Lusis, AJ & Hazen, SL 2013, 'Intestinal microbiota metabolism of l-carnitine, a nutrient in red meat, promotes atherosclerosis', Nature Medicine, vol. 19, no. 5, pp. 576-585. https://doi.org/10.1038/nm.3145
Koeth, Robert A. ; Wang, Zeneng ; Levison, Bruce S. ; Buffa, Jennifer A. ; Org, Elin ; Sheehy, Brendan T. ; Britt, Earl B. ; Fu, Xiaoming ; Wu, Yuping ; Li, Lin ; Smith, Jonathan D. ; Didonato, Joseph A. ; Chen, Jun ; Li, Hongzhe ; Wu, Gary D. ; Lewis, James D. ; Warrier, Manya ; Brown, J. Mark ; Krauss, Ronald M. ; Tang, W. H Wilson ; Bushman, Frederic D. ; Lusis, Aldons J. ; Hazen, Stanley L. / Intestinal microbiota metabolism of l-carnitine, a nutrient in red meat, promotes atherosclerosis. In: Nature Medicine. 2013 ; Vol. 19, No. 5. pp. 576-585.
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