Intestinal calcium transport and calcium extrusion processes at the basolateral membrane

R. H. Wasserman, J. S. Chandler, S. A. Meyer, C. A. Smith, M. E. Brindak, C. S. Fullmer, J. T. Penniston, R. Kumar

Research output: Contribution to journalArticlepeer-review

102 Scopus citations

Abstract

The intestinal absorption of calcium has been proposed to occur by the transcellular transfer of Ca2+ through the enterocyte proper and between the cells of the intestinal epithelium, i.e., the paracellular path. Attention in this report is given to the transcellular models of Ca2+ absorption and, more specifically, the Ca2+ extrusion events occurring at the basolateral membrane. These extrusion processes include the operation of an ATP-dependent Ca2+ pump and a Na+/Ca2+ exchanger, as well as exocytosis as the terminal event in a proposed vesicular transport mechanism. Evidence for the presence of an ATP-dependent Ca2+ pump at the basolateral membrane is documented and illustrated with biochemical and immunological data from studies on the avian intestinal basolateral membrane. As shown immunohistochemically, the Ca2+ pump was primarily localized on the enterocyte basolateral membrane. The ATP-dependency and vitamin D enhancement of Ca2+ uptake by isolated basolateral membrane vesicles are shown. Western blot analysis of intestinal mucosa, by using a monoclonal antibody produced against the erythrocyte Ca2+ pump, indicated that the number of pump units is increased by 1,25-dihydroxycholecalciferol. The possible involvement of calbindin-D(28K) as a direct stimulator of the Ca2+ pump is discussed, and the quantitative relationship between Ca2+ transport rates and Ca2+ pumping activity has been estimated. Information related to the basolateral membrane Na+/Ca2+ exchanger and the vesicular transport model of Ca2+ absorption is also briefly reviewed.

Original languageEnglish (US)
Pages (from-to)662-671
Number of pages10
JournalJournal of Nutrition
Volume122
Issue number3 SUPPL.
DOIs
StatePublished - 1992

Keywords

  • basolateral membrane
  • calcium
  • calcium pump
  • intestine
  • transport

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

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