Interphase chromosome profiling a method for conventional banded chromosome analysis using interphase nuclei

Ramesh Babu, Daniel L. Van Dyke, Vaithilingam G. Dev, Prasad Koduru, Nagesh Rao, Navnit S. Mitter, Mingya Liu, Ernesto Fuentes, Sarah Fuentes, Stephen Papa

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Context.-Chromosome analysis on bone marrow or peripheral blood samples fails in a small proportion of attempts. A method that is more reliable, with similar or better resolution, would be a welcome addition to the armamentarium of the cytogenetics laboratory. Objective.-To develop a method similar to banded metaphase chromosome analysis that relies only on interphase nuclei. Design.-To label multiple targets in an equidistant fashion along the entire length of each chromosome, including landmark subtelomere and centromere regions. Each label so generated by using cloned bacterial artificial chromosome probes is molecularly distinct with unique spectral characteristics, so the number and position of the labels can be tracked to identify chromosome abnormalities. Results.-Interphase chromosome profiling (ICP) demonstrated results similar to conventional chromosome analysis and fluorescence in situ hybridization in 55 previously studied cases and obtained useful ICP chromosome analysis results on another 29 cases in which conventional methods failed. Conclusions.-ICP is a new and powerful method to karyotype peripheral blood and bone marrow aspirate preparations without reliance on metaphase chromosome preparations. It will be of particular value for cases with a failed conventional analysis or when a fast turnaround time is required.

Original languageEnglish (US)
Pages (from-to)213-228
Number of pages16
JournalArchives of Pathology and Laboratory Medicine
Volume142
Issue number2
DOIs
StatePublished - Feb 1 2018

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Medical Laboratory Technology

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    Babu, R., Van Dyke, D. L., Dev, V. G., Koduru, P., Rao, N., Mitter, N. S., Liu, M., Fuentes, E., Fuentes, S., & Papa, S. (2018). Interphase chromosome profiling a method for conventional banded chromosome analysis using interphase nuclei. Archives of Pathology and Laboratory Medicine, 142(2), 213-228. https://doi.org/10.5858/arpa.2016-0621-OA