TY - JOUR
T1 - Interphase chromosome profiling a method for conventional banded chromosome analysis using interphase nuclei
AU - Babu, Ramesh
AU - Van Dyke, Daniel L.
AU - Dev, Vaithilingam G.
AU - Koduru, Prasad
AU - Rao, Nagesh
AU - Mitter, Navnit S.
AU - Liu, Mingya
AU - Fuentes, Ernesto
AU - Fuentes, Sarah
AU - Papa, Stephen
PY - 2018/2/1
Y1 - 2018/2/1
N2 - Context.-Chromosome analysis on bone marrow or peripheral blood samples fails in a small proportion of attempts. A method that is more reliable, with similar or better resolution, would be a welcome addition to the armamentarium of the cytogenetics laboratory. Objective.-To develop a method similar to banded metaphase chromosome analysis that relies only on interphase nuclei. Design.-To label multiple targets in an equidistant fashion along the entire length of each chromosome, including landmark subtelomere and centromere regions. Each label so generated by using cloned bacterial artificial chromosome probes is molecularly distinct with unique spectral characteristics, so the number and position of the labels can be tracked to identify chromosome abnormalities. Results.-Interphase chromosome profiling (ICP) demonstrated results similar to conventional chromosome analysis and fluorescence in situ hybridization in 55 previously studied cases and obtained useful ICP chromosome analysis results on another 29 cases in which conventional methods failed. Conclusions.-ICP is a new and powerful method to karyotype peripheral blood and bone marrow aspirate preparations without reliance on metaphase chromosome preparations. It will be of particular value for cases with a failed conventional analysis or when a fast turnaround time is required.
AB - Context.-Chromosome analysis on bone marrow or peripheral blood samples fails in a small proportion of attempts. A method that is more reliable, with similar or better resolution, would be a welcome addition to the armamentarium of the cytogenetics laboratory. Objective.-To develop a method similar to banded metaphase chromosome analysis that relies only on interphase nuclei. Design.-To label multiple targets in an equidistant fashion along the entire length of each chromosome, including landmark subtelomere and centromere regions. Each label so generated by using cloned bacterial artificial chromosome probes is molecularly distinct with unique spectral characteristics, so the number and position of the labels can be tracked to identify chromosome abnormalities. Results.-Interphase chromosome profiling (ICP) demonstrated results similar to conventional chromosome analysis and fluorescence in situ hybridization in 55 previously studied cases and obtained useful ICP chromosome analysis results on another 29 cases in which conventional methods failed. Conclusions.-ICP is a new and powerful method to karyotype peripheral blood and bone marrow aspirate preparations without reliance on metaphase chromosome preparations. It will be of particular value for cases with a failed conventional analysis or when a fast turnaround time is required.
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U2 - 10.5858/arpa.2016-0621-OA
DO - 10.5858/arpa.2016-0621-OA
M3 - Article
AN - SCOPUS:85045185056
SN - 0003-9985
VL - 142
SP - 213
EP - 228
JO - Archives of Pathology and Laboratory Medicine
JF - Archives of Pathology and Laboratory Medicine
IS - 2
ER -