Intermittent sub-ambient interstitial hydrostatic pressure as a potential mechanical stimulator for chondrocyte metabolism

Jun Kyo Suh, Goo H. Baek, Asbjørn Arøen, Christina M. Malin, Christopher Niyibizi, Christopher H Evans, Andrea Westerhausen-Larson

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Objective: Experimental findings have suggested that the metabolic activities of articular cartilage can be influenced by mechanical stimuli. Our mathematical analysis predicted that cyclic compressive loading may create periods of intermittent sub-ambient hydrostatic pressure within the cartilage extracellular matrix. Based on this mathematical analysis, the present study was aimed to investigate whether the intermittent sub-ambient hydrostatic pressure, created in the cartilage extracellular matrix during cyclic compression, has a stimulative effect on the biosynthesis of chondrocytes. Method: In order to test this hypothesiS, the present study developed a custom-designed sub-ambient pressure generator to subject a monolayer culture of chondrocytes to an intermittent sub-ambient pressure. Using this pressure generator, the monolayer chondrocyte culture system was analyzed for 35S-sulfate and 3H-proline incorporation rates for biosynthesis of proteoglycan and collagenous/noncollagenous protein molecules, respectively. Northern analyses for aggrecan and type II collagen mRNAs were also performed. Results: It was found that the intermittent sub- ambient pressure produced a 40% increase in proteoglycan and a 17% increase in non-collagenous protein synthesis during the pressurization period (P<0.05). The collagenous protein synthesis was not affected by the intermittent sub-ambient pressure regimen used in this study. After the intermittent sub-ambient pressurization, the metabolic activities of the chondrocytes returned to normal (control level). The intermittent sub- ambient pressure also produced an increase in the mRNA signals for aggrecan. Therefore, we conclude that intermittent sub-ambient pressure may be one of the potential mechanical stimulators of chondrocytes in articular cartilage during dynamic compression.

Original languageEnglish (US)
Pages (from-to)71-80
Number of pages10
JournalOsteoarthritis and Cartilage
Volume7
Issue number1
DOIs
StatePublished - Jan 1999
Externally publishedYes

Fingerprint

Hydrostatic Pressure
Hydrostatic pressure
Chondrocytes
Metabolism
Pressure
Cartilage
Aggrecans
Pressurization
Biosynthesis
Articular Cartilage
Proteoglycans
Proteins
Extracellular Matrix
Monolayers
Messenger RNA
Collagen Type II
Level control
Fourier Analysis
Collagen
Proline

Keywords

  • Articular cartilage
  • Cartilage metabolism
  • Chondrocyte
  • Hydrostatic pressure

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Cite this

Intermittent sub-ambient interstitial hydrostatic pressure as a potential mechanical stimulator for chondrocyte metabolism. / Suh, Jun Kyo; Baek, Goo H.; Arøen, Asbjørn; Malin, Christina M.; Niyibizi, Christopher; Evans, Christopher H; Westerhausen-Larson, Andrea.

In: Osteoarthritis and Cartilage, Vol. 7, No. 1, 01.1999, p. 71-80.

Research output: Contribution to journalArticle

Suh, Jun Kyo ; Baek, Goo H. ; Arøen, Asbjørn ; Malin, Christina M. ; Niyibizi, Christopher ; Evans, Christopher H ; Westerhausen-Larson, Andrea. / Intermittent sub-ambient interstitial hydrostatic pressure as a potential mechanical stimulator for chondrocyte metabolism. In: Osteoarthritis and Cartilage. 1999 ; Vol. 7, No. 1. pp. 71-80.
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