Interleukin-2-induced tyrosine phosphorylation of p52shc in T lymphocytes

Leigh Ann Burns, Larry M Karnitz, Shari L. Sutor, Robert T. Abraham

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Stimulation of activated T cells with interleukin-2 (IL-2) results in the tyrosine phosphorylation of several intracellular proteins. The present studies demonstrate that IL-2 stimulation induces phosphorylation of the src homology 2 domain-containing protein, p52shc, on both tyrosine and serine residues. The level of p52shc phosphorylation was maximal within 5 min after growth factor addition and declined gradually thereafter. In addition, anti-Shc immunoprecipitates from IL-2-stimulated T cells contained a co-precipitating protein tyrosine kinase (PTK) activity that phosphorylated p52shc on tyrosine residues in immune complex kinase assays. These results demonstrate that p52shc is an early substrate for IL-2 receptor-coupled PTK activity(s) and suggest that this protein may be involved in the transduction of PTK-dependent regulatory signals in IL-2-stimulated T cells.

Original languageEnglish (US)
Pages (from-to)17659-17661
Number of pages3
JournalJournal of Biological Chemistry
Volume268
Issue number24
StatePublished - Aug 25 1993

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Phosphorylation
T-cells
Interleukin-2
Tyrosine
Protein-Tyrosine Kinases
T-Lymphocytes
Proteins
src Homology Domains
Interleukin-2 Receptors
Receptor Protein-Tyrosine Kinases
Antigen-Antibody Complex
Serine
Assays
Intercellular Signaling Peptides and Proteins
Phosphotransferases
2-tyrosine
Substrates

ASJC Scopus subject areas

  • Biochemistry

Cite this

Interleukin-2-induced tyrosine phosphorylation of p52shc in T lymphocytes. / Burns, Leigh Ann; Karnitz, Larry M; Sutor, Shari L.; Abraham, Robert T.

In: Journal of Biological Chemistry, Vol. 268, No. 24, 25.08.1993, p. 17659-17661.

Research output: Contribution to journalArticle

Burns, LA, Karnitz, LM, Sutor, SL & Abraham, RT 1993, 'Interleukin-2-induced tyrosine phosphorylation of p52shc in T lymphocytes', Journal of Biological Chemistry, vol. 268, no. 24, pp. 17659-17661.
Burns, Leigh Ann ; Karnitz, Larry M ; Sutor, Shari L. ; Abraham, Robert T. / Interleukin-2-induced tyrosine phosphorylation of p52shc in T lymphocytes. In: Journal of Biological Chemistry. 1993 ; Vol. 268, No. 24. pp. 17659-17661.
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