Interlaboratory comparison of IDH mutation detection

Martin J. Van Den Bent, C. Hartmann, Matthias Preusser, Thomas Ströbel, Hendrikus J. Dubbink, Johan M. Kros, Andreas Von Deimling, Blandine Boisselier, Marc Sanson, Kevin C. Halling, Kristin L. Diefes, Kenneth Aldape, Caterina Giannini

Research output: Contribution to journalReview article

46 Scopus citations

Abstract

Isocitrate dehydrogenase (IDH) mutational testing is becoming increasingly important. For this, robust and reliable assays are needed. We tested the variation of results between six laboratories of testing for IDH mutations. Each laboratory received five unstained slides from 31 formalin-fixed paraffin-embedded (FFPE) glioma samples, and followed its own standard IDH diagnostic routine. All laboratories used immunohistochemistry (IHC) with an antibody against the most frequent IDH1 mutation (R132H) as a first step. Three laboratories then sequenced only IHC negative cases while the others sequenced all cases. Based on the overall analysis, 13 samples from 11 tumors had an R132H mutation and one tumor showed an R132G mutation. Results of IHC for IDH1 R132H mutations in all six laboratories were completely in agreement, and identified all R132H mutations. Upon sequencing the results of two laboratories deviated from those of the others. After a review of the entire diagnostic process, on repeat (blinded) testing one laboratory was completely in agreement with the overall result. A change in technique did only partially improve the results in the other laboratory. IHC for the IDH1 R132H mutation is very reliable and consistent across laboratories. IDH sequencing procedures yielded inconsistent results in 2 out of 6 laboratories. Quality assurance is pivotal before IDH testing is made part of clinical management of patients.

Original languageEnglish (US)
Pages (from-to)173-178
Number of pages6
JournalJournal of neuro-oncology
Volume112
Issue number2
DOIs
StatePublished - Apr 1 2013

Keywords

  • IDH1
  • Immunohistochemistry
  • Interlaboratory comparison
  • Sequencing

ASJC Scopus subject areas

  • Oncology
  • Neurology
  • Clinical Neurology
  • Cancer Research

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  • Cite this

    Van Den Bent, M. J., Hartmann, C., Preusser, M., Ströbel, T., Dubbink, H. J., Kros, J. M., Von Deimling, A., Boisselier, B., Sanson, M., Halling, K. C., Diefes, K. L., Aldape, K., & Giannini, C. (2013). Interlaboratory comparison of IDH mutation detection. Journal of neuro-oncology, 112(2), 173-178. https://doi.org/10.1007/s11060-013-1056-z