Interferon-α is a potent inhibitor of basic fibroblast growth factor-stimulated cell proliferation in human uterine cells

Byung Seok Lee, Elizabeth A. Stewart, Marine Sahakian, Romana A. Nowak

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

PROBLEM: Abnormal uterine bleeding is a significant health problem for many women and is the number-one reason for performing hysterectomy in the United States. Leiomyomas (uterine fibroids) are benign neoplams that are a frequent cause of abnormal uterine bleeding. The goal of this study was to assess the effects of the anti-angiogenic cytokine, interferon (INF)-α, on the proliferation of both leiomyoma and normal uterine cells. METHOD OF STUDY: Primary cultures of leiomyoma, myometrial, and endometrial stromal cells were established for in vitro study. The effects of INF-α (10, 100, and 1000 U/ml) were tested on serum-stimulated and basic fibroblast growth factor-stimulated cell proliferation using the [3H]thymidine incorporation assay. RESULTS: INF-α was a potent inhibitor of cell proliferation for all three cell types, with endometrial stromal cells showing the greatest sensitivity. The antiproliferative effect did not appear to result from toxic effects on the cells. CONCLUSION: INFs may prove to be useful therapeutic agents for the treatment of leiomyoma-related abnormal uterine bleeding.

Original languageEnglish (US)
Pages (from-to)19-25
Number of pages7
JournalAmerican Journal of Reproductive Immunology
Volume40
Issue number1
DOIs
StatePublished - Jan 1 1998

Keywords

  • Abnormal uterine bleeding
  • Angiogenesis
  • Cytokine
  • Endometrium
  • Leiomyoma

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Reproductive Medicine
  • Obstetrics and Gynecology

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