Interferences in hormone immunoassays

George G. Klee

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Despite the numerous potential interferences that were discussed in this article, immunoassays, in general, are robust measurement systems. There is no practical way to identify specimens a priori that are likely to have immunoassay interference. Therefore, laboratories must rely on communication from clinicians to identify suspicious test values that may be caused by assay interference. After laboratories are alerted, multiple investigations can be undertaken. The common causes of immunoassay interferences (see Box 1) are dependent on the analytic format. Competitive assays are most likely to have problems with cross-reactivity, whereas immunometric assays most often have problems with heterophile antibodies. The simple steps to test for dilutional linearity, spiked recovery, heterophile antibody blocking, and testing with alternate technology can help in the evaluations of cases that are suspected to have assay interference.

Original languageEnglish (US)
Pages (from-to)1-18
Number of pages18
JournalClinics in Laboratory Medicine
Volume24
Issue number1
DOIs
StatePublished - Mar 2004

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Immunoassay
Heterophile Antibodies
Assays
Hormones
Exercise Test
Technology
Recovery
Communication
Testing

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Interferences in hormone immunoassays. / Klee, George G.

In: Clinics in Laboratory Medicine, Vol. 24, No. 1, 03.2004, p. 1-18.

Research output: Contribution to journalArticle

Klee, George G. / Interferences in hormone immunoassays. In: Clinics in Laboratory Medicine. 2004 ; Vol. 24, No. 1. pp. 1-18.
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