Interactions of NK cell receptor KIR3DL1*004 with chaperones and conformation-specific antibody reveal a functional folded state as well as predominant intracellular retention

Sabrina B. Taner, Marcelo J. Pando, Allison Roberts, Jennifer Schellekens, Steven G E Marsh, Karl Johan Malmberg, Peter Parham, Frances M. Brodsky

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Variable interaction between the Bw4 epitope of HLA-B and the polymorphic KIR3DL1/S1 system of inhibitory and activating NK cell receptors diversifies the development, repertoire formation, and response of human NK cells. KIR3DL1*004, a common KIR3DL1 allotype, in combination with Bw4 + HLA-B, slows progression of HIV infection to AIDS. Analysis in this study of KIR3DL1*004 membrane traffic in NK cells shows this allotype is largely misfolded but stably retained in the endoplasmic reticulum, where it binds to the chaperone calreticulin and does not induce the unfolded protein response. A small fraction of KIR3DL1*004 folds correctly and leaves the endoplasmic reticulum to be expressed on the surface of primary NK and transfected NKL cells, in a form that can be triggered to inhibit NK cell activation and secretion of IFN-γ. Consistent with this small proportion of correctly folded molecules, trace amounts of MHC class I coimmunoprecipitated with KIR3DL1*004. There was no indication of any extensive intracellular interaction between unfolded KIR3DL1*004 and cognate Bw4+ HLA-B. A similarly limited interaction of Bw4 with KIR3DL1*002, when both were expressed by the same cell, was observed despite the efficient folding of KIR3DL1*002 and its abundance on the NK cell surface. Several positions of polymorphism modulate KIR3DL1 abundance at the cell surface, differences that do not necessarily correlate with the potency of allotype function. In this context, our results suggest the possibility that the effect of Bw4+ HLA-B and KIR3DL1*004 in slowing progression to AIDS is mediated by interaction of Bw4+ HLA-B with the small fraction of cell surface KIR3DL1*004.

Original languageEnglish (US)
Pages (from-to)62-72
Number of pages11
JournalJournal of Immunology
Volume186
Issue number1
DOIs
StatePublished - Jan 1 2011
Externally publishedYes

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Natural Killer Cell Receptors
HLA-B Antigens
Natural Killer Cells
Antibodies
Endoplasmic Reticulum
Acquired Immunodeficiency Syndrome
Calreticulin
Unfolded Protein Response
HIV Infections
Epitopes
Membranes

ASJC Scopus subject areas

  • Immunology

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Interactions of NK cell receptor KIR3DL1*004 with chaperones and conformation-specific antibody reveal a functional folded state as well as predominant intracellular retention. / Taner, Sabrina B.; Pando, Marcelo J.; Roberts, Allison; Schellekens, Jennifer; Marsh, Steven G E; Malmberg, Karl Johan; Parham, Peter; Brodsky, Frances M.

In: Journal of Immunology, Vol. 186, No. 1, 01.01.2011, p. 62-72.

Research output: Contribution to journalArticle

Taner, Sabrina B. ; Pando, Marcelo J. ; Roberts, Allison ; Schellekens, Jennifer ; Marsh, Steven G E ; Malmberg, Karl Johan ; Parham, Peter ; Brodsky, Frances M. / Interactions of NK cell receptor KIR3DL1*004 with chaperones and conformation-specific antibody reveal a functional folded state as well as predominant intracellular retention. In: Journal of Immunology. 2011 ; Vol. 186, No. 1. pp. 62-72.
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abstract = "Variable interaction between the Bw4 epitope of HLA-B and the polymorphic KIR3DL1/S1 system of inhibitory and activating NK cell receptors diversifies the development, repertoire formation, and response of human NK cells. KIR3DL1*004, a common KIR3DL1 allotype, in combination with Bw4 + HLA-B, slows progression of HIV infection to AIDS. Analysis in this study of KIR3DL1*004 membrane traffic in NK cells shows this allotype is largely misfolded but stably retained in the endoplasmic reticulum, where it binds to the chaperone calreticulin and does not induce the unfolded protein response. A small fraction of KIR3DL1*004 folds correctly and leaves the endoplasmic reticulum to be expressed on the surface of primary NK and transfected NKL cells, in a form that can be triggered to inhibit NK cell activation and secretion of IFN-γ. Consistent with this small proportion of correctly folded molecules, trace amounts of MHC class I coimmunoprecipitated with KIR3DL1*004. There was no indication of any extensive intracellular interaction between unfolded KIR3DL1*004 and cognate Bw4+ HLA-B. A similarly limited interaction of Bw4 with KIR3DL1*002, when both were expressed by the same cell, was observed despite the efficient folding of KIR3DL1*002 and its abundance on the NK cell surface. Several positions of polymorphism modulate KIR3DL1 abundance at the cell surface, differences that do not necessarily correlate with the potency of allotype function. In this context, our results suggest the possibility that the effect of Bw4+ HLA-B and KIR3DL1*004 in slowing progression to AIDS is mediated by interaction of Bw4+ HLA-B with the small fraction of cell surface KIR3DL1*004.",
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AU - Malmberg, Karl Johan

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