Interactions between nebulin-like motifs and thin filament regulatory proteins

Ozgur Ogut, M. Moazzem Hossain, Jian Ping Jin

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Nebulin (600-900 kDa) and nebulette (107-109 kDa) are two homologous thin filament-associated proteins in skeletal and cardiac muscles, respectively. Both proteins are capped with a unique region at the amino terminus as well as a serine-rich linker domain and SH3 domains at the COOH terminus. Their significant size difference is attributed to the length of the central region wherein both proteins are primarily composed of ∼35 amino acid repeats termed nebulin-like repeats or motifs. These motifs are marked by a conserved SXXXY sequence and high affinity binding to F-actin. To further characterize the effects that nebulin-like proteins may have on the striated muscle thin filament, we have cloned, expressed, and purified a five-motif chicken nebulette fragment and tested its interaction with the thin filament regulatory proteins. Both tropomyosin and troponin T individually bound the nebulette fragment, although the affinity of this interaction was significantly increased when tropomyosin-troponin T was tested as a binary complex. The addition of troponin I to the tropomyosin-troponin T complex decreased the binding to the nebulette fragment, indicating an involvement of the conserved T2 region of troponin T in this interaction. F-actin cosedimentation demonstrated that the nebulette fragment was able to significantly increase the affinity of the tropomyosin-troponin assembly for F-actin. The relationships provide a means for nebulin-like motifs to participate in the allosteric regulation of striated muscle contraction.

Original languageEnglish (US)
Pages (from-to)3089-3097
Number of pages9
JournalJournal of Biological Chemistry
Volume278
Issue number5
DOIs
StatePublished - Jan 31 2003
Externally publishedYes

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Tropomyosin
Troponin T
Muscle
Actins
Troponin
Striated Muscle
Proteins
Allosteric Regulation
Troponin I
src Homology Domains
Conserved Sequence
Muscle Contraction
Serine
Chickens
Myocardium
Skeletal Muscle
nebulin
Amino Acids

ASJC Scopus subject areas

  • Biochemistry

Cite this

Interactions between nebulin-like motifs and thin filament regulatory proteins. / Ogut, Ozgur; Hossain, M. Moazzem; Jin, Jian Ping.

In: Journal of Biological Chemistry, Vol. 278, No. 5, 31.01.2003, p. 3089-3097.

Research output: Contribution to journalArticle

Ogut, Ozgur ; Hossain, M. Moazzem ; Jin, Jian Ping. / Interactions between nebulin-like motifs and thin filament regulatory proteins. In: Journal of Biological Chemistry. 2003 ; Vol. 278, No. 5. pp. 3089-3097.
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