Interaction of measles virus vectors with Auger electron emitting radioisotopes

David Dingli, Kah Whye Peng, Mary E. Harvey, Sompong Vongpunsawad, Elizabeth R. Bergert, Robert A. Kyle, Roberto Cattaneo, John C. Morris, Stephen J. Russell

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

A recombinant measles virus (MV) expressing the sodium iodide symporter (NIS) is being considered for therapy of advanced multiple myeloma. Auger electrons selectively damage cells in which the isotope decays. We hypothesized that the Auger electron emitting isotope 125I can be used to control viral proliferation. MV was engineered to express both carcinoembryonic antigen and NIS (MV-NICE). Cells were infected with MV-NICE and exposed to 125I with appropriate controls. MV-NICE replication in vitro is inhibited by the selective uptake of 125I by cells expressing NIS. Auger electron damage is partly mediated by free radicals and abrogated by glutathione. In myeloma xenografts, control of MV-NICE with 125I was not possible under the conditions of the experiment. MV-NICE does not replicate faster in the presence of radiation. Auger electron emitting isotopes effectively stop propagation of MV vectors expressing NIS in vitro. Additional work is necessary to translate these observations in vivo.

Original languageEnglish (US)
Pages (from-to)22-29
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume337
Issue number1
DOIs
StatePublished - Nov 11 2005

Keywords

  • Auger electron
  • Iodide
  • Multiple myeloma
  • Sodium iodide symporter
  • Virotherapy

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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