Interaction between living bone particles and rhBMP-2 in large segmental defect healing in the rat femur

Fangjun Liu, James W. Wells, Ryan M. Porter, Vaida Glatt, Zhenxin Shen, Martina Schinhan, Alan Ivkovic, Mark S. Vrahas, Christopher H Evans, Elisabeth Ferreira

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Orthopedic surgeons sometimes combine recombinant, human BMP-2 with autograft bone when dealing with problematic osseous fractures. Although some case reports indicate success with this off-label strategy, there have been no randomized controlled trials. Moreover, a literature search revealed only one pre-clinical study and this was in a cranial defect model. The present project examined the consequences of combining BMP-2 with particles of living bone in a rat femoral defect model. Human bone particles were recovered with a reamer-irrigator-aspirator (RIA). To allow acceptance of the xenograft as surrogate autograft, rats were administered an immunosuppressive cocktail that does not interfere with bone healing. Implantation of 200μg living bone particles generated a small amount of new bone and defects did not heal. Graded amounts of BMP-2 that alone provoked no healing (1.1μg), borderline healing (5.5μg), or full healing (11μg) were added to this amount of bone particles. Addition of BMP-2 (1.1μg) increased osteogenesis, and produced bridging in 2 of 7 defects. The combination of BMP-2 (5.5μg) and bone particles made healing more reliable and advanced the maturation of the regenerate. Bone formation with BMP-2 (11μg) and bone particles showed improved maturation. Thus, the combination of autograft and BMP-2 may be helpful clinically under conditions where the healing response is suboptimal.

Original languageEnglish (US)
JournalJournal of Orthopaedic Research
DOIs
StateAccepted/In press - 2016

Fingerprint

Femur
Bone and Bones
Autografts
Osteogenesis
Immunosuppressive Agents
Thigh
Heterografts
Randomized Controlled Trials

Keywords

  • Autograft
  • Immunosuppression
  • INFUSE
  • RIA
  • Xenograft

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Cite this

Liu, F., Wells, J. W., Porter, R. M., Glatt, V., Shen, Z., Schinhan, M., ... Ferreira, E. (Accepted/In press). Interaction between living bone particles and rhBMP-2 in large segmental defect healing in the rat femur. Journal of Orthopaedic Research. https://doi.org/10.1002/jor.23255

Interaction between living bone particles and rhBMP-2 in large segmental defect healing in the rat femur. / Liu, Fangjun; Wells, James W.; Porter, Ryan M.; Glatt, Vaida; Shen, Zhenxin; Schinhan, Martina; Ivkovic, Alan; Vrahas, Mark S.; Evans, Christopher H; Ferreira, Elisabeth.

In: Journal of Orthopaedic Research, 2016.

Research output: Contribution to journalArticle

Liu, Fangjun ; Wells, James W. ; Porter, Ryan M. ; Glatt, Vaida ; Shen, Zhenxin ; Schinhan, Martina ; Ivkovic, Alan ; Vrahas, Mark S. ; Evans, Christopher H ; Ferreira, Elisabeth. / Interaction between living bone particles and rhBMP-2 in large segmental defect healing in the rat femur. In: Journal of Orthopaedic Research. 2016.
@article{be3b97942c6d4caa9603f85466fef0ef,
title = "Interaction between living bone particles and rhBMP-2 in large segmental defect healing in the rat femur",
abstract = "Orthopedic surgeons sometimes combine recombinant, human BMP-2 with autograft bone when dealing with problematic osseous fractures. Although some case reports indicate success with this off-label strategy, there have been no randomized controlled trials. Moreover, a literature search revealed only one pre-clinical study and this was in a cranial defect model. The present project examined the consequences of combining BMP-2 with particles of living bone in a rat femoral defect model. Human bone particles were recovered with a reamer-irrigator-aspirator (RIA). To allow acceptance of the xenograft as surrogate autograft, rats were administered an immunosuppressive cocktail that does not interfere with bone healing. Implantation of 200μg living bone particles generated a small amount of new bone and defects did not heal. Graded amounts of BMP-2 that alone provoked no healing (1.1μg), borderline healing (5.5μg), or full healing (11μg) were added to this amount of bone particles. Addition of BMP-2 (1.1μg) increased osteogenesis, and produced bridging in 2 of 7 defects. The combination of BMP-2 (5.5μg) and bone particles made healing more reliable and advanced the maturation of the regenerate. Bone formation with BMP-2 (11μg) and bone particles showed improved maturation. Thus, the combination of autograft and BMP-2 may be helpful clinically under conditions where the healing response is suboptimal.",
keywords = "Autograft, Immunosuppression, INFUSE, RIA, Xenograft",
author = "Fangjun Liu and Wells, {James W.} and Porter, {Ryan M.} and Vaida Glatt and Zhenxin Shen and Martina Schinhan and Alan Ivkovic and Vrahas, {Mark S.} and Evans, {Christopher H} and Elisabeth Ferreira",
year = "2016",
doi = "10.1002/jor.23255",
language = "English (US)",
journal = "Journal of Orthopaedic Research",
issn = "0736-0266",
publisher = "John Wiley and Sons Inc.",

}

TY - JOUR

T1 - Interaction between living bone particles and rhBMP-2 in large segmental defect healing in the rat femur

AU - Liu, Fangjun

AU - Wells, James W.

AU - Porter, Ryan M.

AU - Glatt, Vaida

AU - Shen, Zhenxin

AU - Schinhan, Martina

AU - Ivkovic, Alan

AU - Vrahas, Mark S.

AU - Evans, Christopher H

AU - Ferreira, Elisabeth

PY - 2016

Y1 - 2016

N2 - Orthopedic surgeons sometimes combine recombinant, human BMP-2 with autograft bone when dealing with problematic osseous fractures. Although some case reports indicate success with this off-label strategy, there have been no randomized controlled trials. Moreover, a literature search revealed only one pre-clinical study and this was in a cranial defect model. The present project examined the consequences of combining BMP-2 with particles of living bone in a rat femoral defect model. Human bone particles were recovered with a reamer-irrigator-aspirator (RIA). To allow acceptance of the xenograft as surrogate autograft, rats were administered an immunosuppressive cocktail that does not interfere with bone healing. Implantation of 200μg living bone particles generated a small amount of new bone and defects did not heal. Graded amounts of BMP-2 that alone provoked no healing (1.1μg), borderline healing (5.5μg), or full healing (11μg) were added to this amount of bone particles. Addition of BMP-2 (1.1μg) increased osteogenesis, and produced bridging in 2 of 7 defects. The combination of BMP-2 (5.5μg) and bone particles made healing more reliable and advanced the maturation of the regenerate. Bone formation with BMP-2 (11μg) and bone particles showed improved maturation. Thus, the combination of autograft and BMP-2 may be helpful clinically under conditions where the healing response is suboptimal.

AB - Orthopedic surgeons sometimes combine recombinant, human BMP-2 with autograft bone when dealing with problematic osseous fractures. Although some case reports indicate success with this off-label strategy, there have been no randomized controlled trials. Moreover, a literature search revealed only one pre-clinical study and this was in a cranial defect model. The present project examined the consequences of combining BMP-2 with particles of living bone in a rat femoral defect model. Human bone particles were recovered with a reamer-irrigator-aspirator (RIA). To allow acceptance of the xenograft as surrogate autograft, rats were administered an immunosuppressive cocktail that does not interfere with bone healing. Implantation of 200μg living bone particles generated a small amount of new bone and defects did not heal. Graded amounts of BMP-2 that alone provoked no healing (1.1μg), borderline healing (5.5μg), or full healing (11μg) were added to this amount of bone particles. Addition of BMP-2 (1.1μg) increased osteogenesis, and produced bridging in 2 of 7 defects. The combination of BMP-2 (5.5μg) and bone particles made healing more reliable and advanced the maturation of the regenerate. Bone formation with BMP-2 (11μg) and bone particles showed improved maturation. Thus, the combination of autograft and BMP-2 may be helpful clinically under conditions where the healing response is suboptimal.

KW - Autograft

KW - Immunosuppression

KW - INFUSE

KW - RIA

KW - Xenograft

UR - http://www.scopus.com/inward/record.url?scp=84964344022&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84964344022&partnerID=8YFLogxK

U2 - 10.1002/jor.23255

DO - 10.1002/jor.23255

M3 - Article

C2 - 27037517

AN - SCOPUS:84964344022

JO - Journal of Orthopaedic Research

JF - Journal of Orthopaedic Research

SN - 0736-0266

ER -