Insulin regulates low density lipoprotein metabolism by swine granulosa cells

Johannes D Veldhuis, J. E. Nestler, J. F. Strauss, J. T. Gwynne

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Insulin synergistically amplified the stimulatory effect of low density lipoprotein (LDL) on progesterone biosynthesis by primary cultures of swine granulosa cells. The mechanisms subserving this facilitate interaction included the following: 1) insulin's synergism with LDL was profoundly attenuated by covalent modification of arginine residues in LDL by 1,2-cyclohexanedione treatment; 2) insulin increased by 2- to 6-fold the number of specific high affinity LDL receptors on granulosa cells, with no change in apparent binding affinity; 3) insulin augmented ratios of [125I]iodo-LDL internalization and degradation without enhancing nonspecific bulk fluid-phase pinocytosis (assessed with [125I]iodo-polyvinylpyrollidone); 4) insulin increased by 2.5 to 3-fold granulosa cell content of free and esterified cholesterol (measured by fluorometry) in response to treatment with unlabeled LDL; 5) insulin stimulated the intracellular accumulation of free [3H]cholesterol and [3H]cholesteryl ester, and amplified [3H]progesterone secretion by granulosa cells exposed to [3H]cholesteryl linoleate-labeled LDL; and 6) insulin action was specific in that it was not mimicked by desoctapeptide insulin, epidermal growth factor, fibroblast growth factor, or relaxin. We conclude that insulin and LDL synergistically enhance progesterone biosynthesis by swine granulosa cells via specific mechanisms that depend upon 1,2-cyclohexanedione-sensitive residues within LDL apoprotein. Insulin action results in significantly augmented binding, internalization, and degradation of LDL, which is accompanied by increased effectual delivery of cholesterol substrate into cellular sterol pools that participate in enhanced steroidogenesis.

Original languageEnglish (US)
Pages (from-to)2242-2253
Number of pages12
JournalEndocrinology
Volume118
Issue number6
StatePublished - 1986
Externally publishedYes

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Granulosa Cells
LDL Lipoproteins
Swine
Insulin
Progesterone
Cholesterol
Pinocytosis
Relaxin
Fluorometry
Apoproteins
Fibroblast Growth Factors
Cholesterol Esters
LDL Receptors
Sterols
Epidermal Growth Factor
Arginine

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Veldhuis, J. D., Nestler, J. E., Strauss, J. F., & Gwynne, J. T. (1986). Insulin regulates low density lipoprotein metabolism by swine granulosa cells. Endocrinology, 118(6), 2242-2253.

Insulin regulates low density lipoprotein metabolism by swine granulosa cells. / Veldhuis, Johannes D; Nestler, J. E.; Strauss, J. F.; Gwynne, J. T.

In: Endocrinology, Vol. 118, No. 6, 1986, p. 2242-2253.

Research output: Contribution to journalArticle

Veldhuis, JD, Nestler, JE, Strauss, JF & Gwynne, JT 1986, 'Insulin regulates low density lipoprotein metabolism by swine granulosa cells', Endocrinology, vol. 118, no. 6, pp. 2242-2253.
Veldhuis JD, Nestler JE, Strauss JF, Gwynne JT. Insulin regulates low density lipoprotein metabolism by swine granulosa cells. Endocrinology. 1986;118(6):2242-2253.
Veldhuis, Johannes D ; Nestler, J. E. ; Strauss, J. F. ; Gwynne, J. T. / Insulin regulates low density lipoprotein metabolism by swine granulosa cells. In: Endocrinology. 1986 ; Vol. 118, No. 6. pp. 2242-2253.
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