Insulin-like growth factor I/somatomedin-C (IGF-I/SM-C) and glucocorticoids synergistically regulate mitosis in competent human fibroblasts

Cheryl A Conover, L. A. Dollar, R. L. Hintz, R. G. Rosenfeld

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Abstract

In serum-free medium, insulin-like growth factor-I/somatomedin-C (IGF-I/SM-C) was weakly mitogenic for adult human fibroblasts in culture. However, in the presence of 0.5% human hypopituitary serum (HHS), which by itself had little effect, there was a marked dose-dependent response to IGF-I/SM-C with a 10- to 20-fold increase in [3H]thymidine incorporation at 25 ng/ml IGF-I/SM-C. With the further addition of dexamethasone or hydrocortisone to the combination of IGF-I/SM-C + 0.5% HHS, there was a dramatic synergistic effect resulting in a 60- to 70-fold increase in [3H]thymidine incorporation. This stimulation was two times greater than that seen with 20% FCS. In contrast, glucocorticoids had no effect in serum-free medium or with HHS alone. These [3H]thymidine incorporation results were clearly supported by cell replication studies. Dose-response curves for 125I IGF-I/SM-C binding and IGF-I/SM-C stimulation of [3H]thymidine incorporation were similar with 1/2 maximal effects for both at 5 ng/ml. However, the striking synergism seen with glucocorticoids occurred in the absence of any glucocorticoid-induced change in IGF-I/SM-C binding, indicating that the interaction of IGF-I/SM-C and glucocorticoids occurs at a postreceptor level. These data demonstrate that in the presence of a low concentration of HHS, IGF-I/SM-C and glucocorticoids stimulate complete cell cycle traverse and replication of human fibroblasts.

Original languageEnglish (US)
Pages (from-to)191-197
Number of pages7
JournalJournal of Cellular Physiology
Volume116
Issue number2
StatePublished - 1983
Externally publishedYes

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Fibroblasts
Insulin-Like Growth Factor I
Mitosis
Glucocorticoids
Thymidine
Serum-Free Culture Media
Serum
Cell culture
Dexamethasone
Hydrocortisone
Cell Cycle
Cells

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

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Insulin-like growth factor I/somatomedin-C (IGF-I/SM-C) and glucocorticoids synergistically regulate mitosis in competent human fibroblasts. / Conover, Cheryl A; Dollar, L. A.; Hintz, R. L.; Rosenfeld, R. G.

In: Journal of Cellular Physiology, Vol. 116, No. 2, 1983, p. 191-197.

Research output: Contribution to journalArticle

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AU - Rosenfeld, R. G.

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